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GO:0006355regulation of transcription, DNA-templatedPMID:20545868IMP: Inferred from Mutant Phenotype P
This annotation made on page: BPMT4:WHIB
By: AHutchison (group Team Purple A 2018) on 2018-03-16 16:03:52 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Public
Assessment
Ivanerill2018-04-14 20:30:20 CDTNo notes given.Acceptable
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2018-03-26 10:18:56 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0006355 regulation of transcription, DNA-templated PMID:20545868 IDA: Inferred from Direct Assay P The protein WhiBTM4 from mycobacteriophage TM4 serves as a transcription regulator in bacteria such as M. smegmatis, in which it inhibits WhiB2. When WhiBTM4 was expressed in M. smegmatis, it caused the cells to lose acid-fast properties and created long, branched cells. WhiBTM4 caused these changes, in which the results were similar to a WhiB2 knockout protein (Fig. 2). When M. smegmatis contained the protein WhiBTM4, WhiB2 mRNA levels (measured using quantitative RT-PCR) greatly decreased compared to the wildtype, which only contained pSD24 (Fig. 3B). A decrease in mRNA levels suggests that the mycobacteriophage TM4 protein WhiBTM4 inhibits transcription of WhiB2 in M. smegmatis. WhiBTM4 binds only to a DNA WhiB2 promoter, which shows that transcription is regulated through DNA (Fig 6E). This is shown by the band created by the protein/DNA complex in figure 6 B-E. complete
CACAO 13120
on BPMT4:WHIB
Flagged
Public
Assessment
Scaruso2018-03-23 10:44:27 CDT

The beginning of your note is background (up through Fig. 2), but you describe Fig 3. correctly. Except that it isn't an IMP, it's an IDA. They added the putative repressor, looked for repression, and got it. Later figures show that it binds to DNA, which justifies your DNA-templated GO code, which you will need to mention if you want to keep it. Otherwise, you will need to back off a step on the GO code.

Requires Changes
Protein
Publication
Qualifier
Evidence
With/From
Notes
Unique/Original