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Cacao

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GO:0044660cytolysis by virus via pore formation in host cell membranePMID:26471254IMP: Inferred from Mutant Phenotype P
This annotation made on page: BPMD2:HOLIN
By: AHutchison (group Team Purple A 2018) on 2018-03-04 20:34:59 CST.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Public
Assessment		Ivanerill2018-04-14 20:26:39 CDTNo notes given.Acceptable
✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
✔ Evidence
✔ With/From
✔ Notes
✔ Unique/Original
Public
Assessment		DanielRenfro2018-03-11 21:02:16 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0044660 cytolysis by virus via pore formation in host cell membrane PMID:26471254 IMP: Inferred from Mutant Phenotype P Mycobacterium phage D29 gene gp11 codes for the protein holin. The protein allows phage D29 to kill both Mycobacterium smegmatis and Escherichia coli by creating pores in the host membrane. The expression of the entire gp11 gene (using vectors) decreased the growth of E. coli significantly (Fig 2A and presence of gp11 confirmed by Western Blot in 2B). The expression of whole gp11 also decreased the growth of M. smegmatis (Fig 2C). Shortened versions Hol68 and Hol93, with amino acids cut off the C-terminal end, did not affect E. coli bacterial growth but Hol116 had a similar reduction in bacterial growth to full length holin protein (Fig. 3B). Figure 3C confirms the presence of holin in all shortened versions and compares them to full length holin. Spot assays confirm the data found in figure 3B because Hol116 showed little to no growth, whereas Hol68 and Hol93 show a significantly larger amount of growth compared to full length holin and Hol116 (Fig. 3D). The spot test of Hol116 also looked very similar to full length holin (Fig. 3D). This indicates that the N-terminal, up through amino acid 116, is important in the function of holin to cause bacterial cell death. The protein must be greater than 93 amino acids long to still contain the function of holin (Fig. 3, all). complete
CACAO 13102
on BPMD2:HOLIN
Flagged
Public
Assessment		Scaruso2018-03-08 10:51:56 CST

Very nice job, but I think you will want to make some small adjustments. The note you have written is describing Fig 2 in nice detail, but since you are going for the IMP, you really want to focus on Fig 3, so you need to add a couple details to that part. Not much, just a bit. Also, you have an error, they determine that somewhere between the first 93 and 116 AAs (N-terminal, not C-) are needed for toxicity.

Requires Changes
✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
✔ Evidence
✔ With/From
✗ Notes
✔ Unique/Original


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