GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
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Cacao
| GO:0034061 | DNA polymerase activity | PMID:27052734 | IDA: Inferred from Direct Assay | F | ||||
| This annotation made on page: 9CAUD:G0YV93 By: Tewell1 (group Team Red-B) on 2017-04-16 18:58:58 CDT. | ||||||||
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| Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment |
|---|---|---|---|---|
| Public Assessment | Scaruso | 2017-05-25 08:43:41 CDT | scaruso changed the notes from "Southern Blot" to "primer extension assay." Otherwise correct. | Updated by Instructor ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✔ Notes ✔ Unique/Original |
| Public Assessment | Scaruso | 2017-05-24 14:55:57 CDT | They did show the DNAP activity, as you describe, but it isn't a Southern Blot. They carried out the extension using 32P labeled primer, then ran the products on a non-denaturing acrylamide gel, and imaged the result. It was never transferred to a membrane and probed, as it would be on a Southern. | Requires Changes ✔ Protein ✔ Publication ✔ Qualifier ✔ Go term ✔ Evidence ✔ With/From ✗ Notes ✔ Unique/Original |
