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Protein Name: RNA-Binding Protein RBM24. Notes: Figures 5G-H and 6D-E are used. First, the former two figures prove that RBM24 destabilizes the mRNA of p63. The paper states that “the half-life of ΔNp63 mRNA was deceased from 3.7 hours in the control cells to 2.7 hours in cells with RBM24 expression, and the half-life of TAp63 mRNA was deceased from 9.3 hours in the control cells to 7.2 hours in cells with RBM24 expression.” As can be seen in the line graphs, the p63 transcripts destabilize more quickly in the presence of RBM24. Thus, RBM24 is linked to mRNA destabilization. It is also linked to 3’UTR-mediated mRNA destabilization in particular, as proven by Figures 6D-E. As the paper states, “we found that RBM24 had no effect on TAp63α expression from an expression vector that only contains TAp63α coding sequence (Fig. 6D). By contrast, TAp63α expression was significantly inhibited by RBM24 when the TAp63α expression vector contains a full-length p63 3′UTR (Fig. 6E).” In Figure 6D, it can be seen that the relative level of TAp63α barely changed when RBM24 was transfected into the cells. However, in Figure 6E, the level decreased significantly, due to the presence of a 3’UTR region. Thus, the inhibition of p63 occurs by the destabilization of its transcripts, specifically the 3’UTR region of mRNA. This links RBM24 to 3’UTR-mediated mRNA destabilization.||complete
CACAO 12379