Cacao

UniProt’s Protein Name: Far upstream element-binding protein 2 OR ALTERNATIVELY KH type-splicing regulatory protein. Organism: Homo sapiens. Notes: Figure 6E is used. Figure 4 had already demonstrated that KSRP downregulates the Spry4 protein, and Figure 5D had already demonstrated that KSRP binds to the 3’-UTR mRNA of the Spry4 gene. In the paper, it is claimed that “if the effects of KSRP on Spry4 3’UTR were specific; then upon KSRP knockdown, the KSRP-mediated mRNA repression should be relaxed, as detected by an increase in luciferase activities. Indeed, knockdown of KSRP increased the luciferase activities of Luc-Spry4 UTR, but not the luciferase activities of Luc-Spry4 UTR-F1.” The chart in Figure 6E does indeed show that luciferase activities approximately double when KSRP is mutated. Thus, KSRP destabilizes the mRNA of Spry4 by binding to the 3’-UTR region.||complete
CACAO 12364