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Cacao

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GO:0043066negative regulation of apoptotic processPMID:28297718IMP: Inferred from Mutant Phenotype P
This annotation made on page: MOUSE:DND1
By: TheBockster (group Team Daytona Bronze Viper) on 2017-03-17 02:00:56 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeCSears,
Team Girl, you trypsin		2017-03-28 14:33:40 CDT

GO:0036098 male germ-line stem cell population maintenance Figure 3E: Mice expressing loss-of-function mutations of DND1 protein showed increased apoptosis and a complete loss of germ-line stem cells, “indicating its critical role in SSC self-renewal”

4
Private
Assessment		Suzialeksander2017-06-13 15:59:49 CDTYou need to be an instructor to view these notes.Unacceptable
Private
Assessment		Suzialeksander2017-05-01 15:17:05 CDTYou need to be an instructor to view these notes.Requires Changes
Public
Assessment		DanielRenfro2017-03-28 14:33:40 CDT

This annotation has been flagged because it has been challenged since last assessment

Flagged
Public
Assessment		DanielRenfro2017-03-25 23:25:12 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0043066 negative regulation of apoptotic process PMID:28297718 IDA: Inferred from Direct Assay P Paper's Protein Name: RNA-binding protein DND1.

UniProt's Protein Name: Dead end protein homolog 1. Organism: Mus musculus (Mouse). Notes: This paper deals with the destabilization of specific mRNA segments by the protein Dnd1. One example of this involves apoptosis. When Dnd1 is mutated and loses function, there is “an upregulation of DND1 PAR-CLIP targets... including positive regulators of apoptosis.” More importantly, Extended figure 6g shows that after GSCs are transduced with DND1’s shRNA targets, cell death occurs within 4 days. The paper also states that “Annexin V-positive cells represent early apoptotic cells and are gated as P8 population (green)” in Extended Figure 6i. As can be seen in the figure, there are clearly more “early apoptotic cells” when the cells in question are transduced with DND1’s shRNA targets. It appears that the process for transduction was done with shRNA targets originating from mice, as is listed in Supplementary Table 9. ||complete
CACAO 12319

on MOUSE:DND1
Flagged
Public
Assessment		DanielRenfro2017-03-21 13:27:26 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0043066 negative regulation of apoptotic process PMID:28297718 IMP: Inferred from Mutant Phenotype P Paper's Protein Name: RNA-binding protein DND1.

UniProt's Protein Name: Dead end protein homolog 1. Organism: Mus musculus (Mouse). Notes: This paper deals with the destabilization of specific mRNA segments by the protein Dnd1. One example of this involves apoptosis. When Dnd1 is mutated and loses function, there is “an upregulation of DND1 PAR-CLIP targets... including positive regulators of apoptosis" This in turn results in “a significant increase in apoptosis.” Thus, this paper suggests a negative relationship between Dnd1 and apoptosis. The figure that supports this conclusion is Extended Data Figure 6m, which shows that positive regulators of apoptosis have a greater increase in abundance when Dnd1 is mutated.||complete
CACAO 12319

on MOUSE:DND1
Flagged
Private
Assessment		Suzialeksander2017-03-20 17:08:53 CDTYou need to be an instructor to view these notes.Requires Changes
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