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Cacao

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GO:0065007biological regulationPMID:4372022IMP: Inferred from Mutant Phenotype P
This annotation made on page: SALTS:A0A0H3NF77
By: Joshuakrank (group Team Moldevort) on 2017-02-26 20:08:33 CST.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeRyancallahan,
Team Three		2017-03-15 16:41:10 CDT

GO: 0044067 - modification by symbiont of host intercellular junctions

Notes: Figure 1 shows that AvrA positively regulates the expression of tight junction proteins such as ZO-1, occludin, and claudin-1. Mutant strains deficient of AvrA showed decreased tight junction stability in the colon of a mouse

2
ChallengeRyancallahan,
Team Three		2017-03-11 13:21:04 CST

Incorrect PMID and changes to GO ID/term name:

PMID: 18523661

GO ID: 1903348

GO term name: positive regulation of bicellular tight junction assembly

Notes: Figure 1 shows that AvrA positively regulates the expression of tight junction proteins such as ZO-1, occludin, and claudin-1. Mutant strains deficient of AvrA showed decreased tight junction stability

0
Private
Assessment		Suzialeksander2017-06-13 14:55:28 CDTYou need to be an instructor to view these notes.Updated by Instructor
Private
Assessment		Suzialeksander2017-05-01 16:41:07 CDTYou need to be an instructor to view these notes.Requires Changes
Public
Assessment		DanielRenfro2017-03-24 15:05:43 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0044067 modification by symbiont of host intercellular junctions PMID:25838979 IMP: Inferred from Mutant Phenotype P Figure 1 (a + b) : The tight junction protein ZO-1 was significantly increased in the presence of wild type S. typhimurium strain SL1344. The mutant strain lacking AvrA experienced a significant reduction of ZO-1 expression in a mouse

Figure 1 (d +e): The AvrA protein was then placed in another S.typhimurium strain (14028). This strain normally doesn't express AvrA nor does it normally impact the tight junction ZO-1 protein. When manipulated to express AvrA, the ZO-1 tight junction protein significantly increased in a mouse.

Fig 1h: Using real time PCR, it was observed that the regulation of ZO-1 was not based on ZO-1 mRNA levels. This is indicative that the AvrA protein is responsible for the up-regulation of the ZO-1 tight junction protein

Fig 4 (a + b): The paper references how intestinal tight junctions seal the paracellular space between epithelial cells and prevent the diffusion of microorganisms and antigens from crossing the epithelium. This figure shows the trans-epithelial resistance of mouse intestinal epithelial cells with bacterial with and without AvrA to observe permeability integrity. Mice infected with AvrA positive S. typhimurium had lower cell permeability than those infected with the AvrA negative mutant. This is indicative that AvrA has a direct impact on intestinal tight junctions and their proteins (like ZO-1)||complete
CACAO 12294

on SALTS:A0A0H3NF77
Flagged
Private
Assessment		Suzialeksander2017-03-24 14:32:31 CDTYou need to be an instructor to view these notes.Requires Changes
✗ Go term
✗ Notes
Public
Assessment		Suzialeksander2017-03-24 14:20:24 CDT

Read my email again!

Requires Changes
✗ Notes
Public
Assessment		DanielRenfro2017-03-24 13:43:12 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0044067 modification by symbiont of host intercellular junctions PMID:25838979 IMP: Inferred from Mutant Phenotype P Figure 1 (a + b) : The tight junction protein ZO-1 was significantly increased in the presence of wild type S. typhimurium strain SL1344. The mutant strain lacking AvrA experienced a significant reduction of ZO-1 expression in a mouse

Figure 1 (d +e): The AvrA protein was then placed in another S.typhimurium strain (14028). This strain normally doesn't express AvrA nor does it normally impact the tight junction ZO-1 protein. When manipulated to express AvrA, the ZO-1 tight junction protein significantly increased in a mouse.

Fig 1h: Using real time PCR, it was observed that the regulation of ZO-1 was not based on ZO-1 mRNA levels. This is indicative that the AvrA protein is responsible for the up-regulation of the ZO-1 tight junction protein

complete
CACAO 12294
on SALTS:A0A0H3NF77
Flagged
Private
Assessment		JimHu2017-03-24 10:58:45 CDTYou need to be an instructor to view these notes.Requires Changes
✔ Publication
✔ Qualifier
✗ Go term
✔ Evidence
✔ With/From
✗ Notes
✔ Unique/Original
Private
Assessment		Suzialeksander2017-03-23 17:00:35 CDTYou need to be an instructor to view these notes.Flagged
Public
Assessment		DanielRenfro2017-03-22 23:52:05 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0044067 modification by symbiont of host intercellular junctions PMID:25838979 IMP: Inferred from Mutant Phenotype P Figure 1: The AvrA protein (from Salmonella typhimurium strain SL1344) expression increases ZO-1 but not alpha-catenin in the colon of a mouse

Figure 3: AvrA regulated the protein expression of ZO-1 through JNK pathway

Figure 5: The effect of AvrA on ZO-1 was eliminated by the presence of JNK inhibitor SP00125||complete
CACAO 12294

on SALTS:A0A0H3NF77
Flagged
Public
Assessment		DanielRenfro2017-03-18 10:46:36 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0065007 biological regulation PMID:25838979 IMP: Inferred from Mutant Phenotype P Figure 1: The AvrA protein (from Salmonella typhimurium strain SL1344) expression increases ZO-1 but not alpha-catenin in the colon of a mouse

Figure 3: AvrA regulated the protein expression of ZO-1 through JNK pathway

Figure 5: The effect of AvrA on ZO-1 was eliminated by the presence of JNK inhibitor SP00125||complete
CACAO 12294

on SALTS:A0A0H3NF77
Flagged
Public
Assessment		DanielRenfro2017-03-15 16:41:10 CDT

This annotation has been flagged because it has been challenged since last assessment

Flagged
Public
Assessment		Suzialeksander2017-03-13 16:08:20 CDT

Challenge term implies Salmonella has tight junctions, but the TJs are in the human host. Look again at the terms.

Requires Changes
Public
Assessment		DanielRenfro2017-03-11 13:21:04 CST

This annotation has been flagged because it has been challenged since last assessment

Flagged
Private
Assessment		Suzialeksander2017-03-10 16:50:25 CSTYou need to be an instructor to view these notes.Requires Changes
✔ Protein
✗ Publication
✔ Qualifier
✗ Go term
✔ Evidence
✔ With/From
✗ Notes
✔ Unique/Original
Private
Assessment		Sandyl 272017-03-07 13:31:00 CSTYou need to be an instructor to view these notes.Flagged


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