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GO:0071076RNA 3' uridylationPMID:18951094IDA: Inferred from Direct Assay P
This annotation made on page: MOUSE:LN28A
By: RazMonk (group Team Gene String) on 2017-02-14 13:54:05 CST.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeSarahCouv,
Team Lily Whale
2017-02-24 15:58:52 CST

The evidence code is incorrect and should be ISM. Also, the corrected notes should be: B) Shown are the U-tail sequences of the up-let-7a-1 cloned from HEK293T cells ectopically expressing pri-let-7a-1 and Lin28a. The U-tails were found in all 28 sequenced clones. MOUSE:LN28A

0
Private
Assessment
JimHu2017-04-23 15:56:07 CDTYou need to be an instructor to view these notes.Acceptable
Protein
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Qualifier
Go term
Evidence
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Notes
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Assessment
DanielRenfro2017-04-23 14:48:56 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0071076 RNA 3' uridylation PMID:18951094 IDA: Inferred from Direct Assay P Fig. 3Aa and 3Ab: When comparing Lin28a mutant with wildtype, it can be seen that Lin28a does bind the miRNA species in question, pri-let-7a. With both pri-let-7a and Lin28a present, the sharp band at pre-let-7a produced with mutant lin28a seen on the northern blot is abrogated and instead a smear can be seen in its place. In Fig. 3Ba, these species of varying lengths (cloned from HEK293T cells ectopically expressing pri-let-7a-1 and Lin28a) are sequenced showing 3' uridylation of an average of 14 U nucleotides.

Called Lin28a in paper||complete
CACAO 12262

on MOUSE:LN28A
Flagged
Public
Assessment
DanielRenfro2017-04-23 14:40:45 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0071076 RNA 3' uridylation PMID:18951094 IDA: Inferred from Direct Assay P Fig. 3Aa and 3Ab: When comparing Lin28a mutant with wildtype, it can be seen that Lin28a does bind the miRNA species in question, pri-let-7a. With both pri-let-7a and Lin28a, the sharp band at pre-let-7a seen on a northern blot is abrogated and instead a smear can be seen in its place either without lin28a or with lin28a mutant. In Fig. 3Ba, these species of varying lengths (cloned from HEK293T cells ectopically expressing pri-let-7a-1 and Lin28a) are sequenced showing 3' uridylation of an average of 14 U nucleotides.

Called Lin28a in paper||complete
CACAO 12262

on MOUSE:LN28A
Flagged
Private
Assessment
JimHu2017-04-22 16:09:44 CDTYou need to be an instructor to view these notes.Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Notes
Unique/Original
Public
Assessment
DanielRenfro2017-04-18 13:09:38 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0071076 RNA 3' uridylation PMID:18951094 IMP: Inferred from Mutant Phenotype P Fig. 3b: long RNA species have 3'-terminal extensions of ~14 nucleotides, most of which are U when incubated with ectopically expressed mouse Lin28a.

Called Lin28a in paper||complete
CACAO 12262

on MOUSE:LN28A
Flagged
Public
Assessment
DanielRenfro2017-04-18 12:56:13 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0071076 RNA 3' uridylation PMID:18951094 IMP: Inferred from Mutant Phenotype P Fig. 3b: long RNA species have 3'-terminal extensions of ~14 nucleotides, most of which are U when incubated with overexpressed mouse Lin28a.

Called Lin28a or collectively as Lin28 in the paper.||complete
CACAO 12262

on MOUSE:LN28A
Flagged
Public
Assessment
DanielRenfro2017-02-28 14:28:27 CST

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0071076 RNA 3' uridylation PMID:18951094 IMP: Inferred from Mutant Phenotype P Fig. 3: long RNA species have 3'-terminal extensions of ~14 nucleotides, most of which are U when incubated with mouse Lin28a.

Called Lin28a or collectively as Lin28 in the paper.||complete
CACAO 12262

on MOUSE:LN28A
Flagged
Public
Assessment
DanielRenfro2017-02-28 14:26:10 CST

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0071076 RNA 3' uridylation PMID:18951094 IDA: Inferred from Direct Assay P Fig. 3: long RNA species have 3'-terminal extensions of ~14 nucleotides, most of which are U when incubated with mouse Lin28a.

Called Lin28a or collectively as Lin28 in the paper.||complete
CACAO 12262

on MOUSE:LN28A
Flagged
Private
Assessment
Suzialeksander2017-02-27 13:38:56 CSTYou need to be an instructor to view these notes.Requires Changes
Public
Assessment
DanielRenfro2017-02-24 15:58:52 CST

This annotation has been flagged because it has been challenged since last assessment

Flagged
Private
Assessment
Suzialeksander2017-02-24 14:33:11 CSTYou need to be an instructor to view these notes.Requires Changes
Publication
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Notes