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GO:0034061DNA polymerase activityPMID:4622590IDA: Inferred from Direct Assay F
This annotation made on page: 9CAUD:A0A024B145
By: Corneljl (group Team Phages Woo!) on 2016-04-11 03:44:16 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeGiesslca,
Team Go Dogs Go
2016-03-30 15:22:55 CDT

Any annotation with Evoli should be a transfer annotation.

None, yet.
ChallengeAdkinssm,
Team Syndicate
2016-03-30 15:05:58 CDT

I do not believe this gene is homologous with the DNA polymerase gene in SPO1. I am not sure how you came to this conclusion, and if you could at least provide some clarity that would be helpful.

I submitted the sequence to HHPred and found that this protein is homologous with Mre11-Rad50, a DNA double-strand break repair protein in Methanocaldococcus jannaschii (found here: http://www.rcsb.org/pdb/explore.do?structureId=3AV0). If you determine this is actually the case, a suitable GO term may be "double-strand break repair"-GO:0006302.

None, yet.
ChallengeAdkinssm,
Team Syndicate
2016-03-30 15:05:56 CDT

I do not believe this gene is homologous with the DNA polymerase gene in SPO1. I am not sure how you came to this conclusion, and if you could at least provide some clarity that would be helpful.

I submitted the sequence to HHPred and found that this protein is homologous with Mre11-Rad50, a DNA double-strand break repair protein in Methanocaldococcus jannaschii (found here: http://www.rcsb.org/pdb/explore.do?structureId=3AV0). If you determine this is actually the case, a suitable GO term may be "double-strand break repair"-GO:0006302.

None, yet.
ChallengeMnguy1,
Team Blue B
2016-03-30 14:25:36 CDT

This reference shows that DNA polymerase activity stays the same after injected to the pol-phage, but after 10 min, there is a huge increase in activity of polymerase within the cell which proved by increasing in DNA synthesis level. The source is more bout DNA polymerase activity as biological process than as a complex - cellular component.

None, yet.
ChallengeHazardb,
Team Snorlax
2016-03-30 10:35:37 CDT

I am challenging this annotation because the phage described in the notes of this annotation (evoli phage) is not the same phage as mentioned in the pubmed paper. The phage mentioned in the paper is named phage SPO1. The paper describes how phage SPO1 infects the mutant pol- bacillus subtilis, but the mutant pol- bacillus subtilis does not include a DNA polymerase. The phage does include a DNA polymerase and when injected into the mutant pol- bacillus subtilis, the phage SPO1 begins to replicate itself, and this can be seen in figure 4 of the paper.

None, yet.
ChallengeE63,
Team Blue A
2016-03-30 09:50:37 CDT

This should be a direct annotation on the species page that the scientists used in the PubMed paper (not to Phage Evoli) and then make another transfer annotation.

2
Private
Assessment
Suzialeksander2016-06-17 13:05:59 CDTYou need to be an instructor to view these notes.Unacceptable
Public
Assessment
JimHu2016-05-02 12:17:00 CDT

Can't make direct annotations to Evoli. Any direct annotations should be made on a different gene.

Requires Changes
Publication
Evidence
Public
Assessment
DanielRenfro2016-04-11 15:44:17 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0034061 DNA polymerase activity PMID:4622590 IDA: Inferred from Direct Assay F The YP address was taken from the Evoli phage in GenBank. The YP address was searched for in UniProt for the accession number. The accession number was searched in Quick Go. This made sure that the gene had not been annotated previously.This is a direct annotation. The GO ID was determined from searching for DNA polymerase in Quick Go. DNA polymerase is an enzyme that aids in DNA synthesis. complete
CACAO 11457
on 9CAUD:A0A024B145
Flagged
Public
Assessment
Suzialeksander2016-04-10 22:38:18 CDT

I didn't read this paper at all, so I cannot assess the annotation, but I caution you all:

  • GO:0042575 DNA polymerase complex: this is a Cellular Component term. It means the annotated protein is shown to be part of the machinery that makes up the polymerase- usually these types of things are shown in gels where they separate all the components of a polymerase complex or something.

This is NOT the same as

  • GO:0034061 DNA polymerase activity: this is a Molecular Function term. It means the annotated protein is actually DOING the synthesis. If they drop the annotated protein in with all the needed "ingredients", they'll probably be measuring the DNA produced by the polymerase.

Compare both the definitions if you're unsure, and remember to FIX your own annotations if you've misused a Component term when you wanted a Function or Process term.

Again, I'm NOT saying this current one is incorrect or not, but this is a commonly misused term.

Flagged