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Cacao

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GO:0005730nucleolusPMID:26967976IDA: Inferred from Direct Assay C
This annotation made on page: HV1H2:GAG
By: Kaylamichbrown (group Team MIA) on 2016-04-12 08:05:48 CDT.



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Team MIA5




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Private
Assessment		Suzialeksander2016-04-22 16:39:10 CDTYou need to be an instructor to view these notes.Acceptable
✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
✔ Evidence
✔ With/From
✔ Notes
✔ Unique/Original
Public
Assessment		DanielRenfro2016-04-12 20:05:48 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005730 nucleolus PMID:26967976 IDA: Inferred from Direct Assay C Figure 1E complete
CACAO 11399
on HV1H2:GAG
Flagged
Public
Assessment		DanielRenfro2016-04-12 16:47:33 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0005730 nucleolus PMID:26967976 IDA: Inferred from Direct Assay C We found that NC localizes throughout the entire cell, including in the nucleus and nucleolus (Fig. 1A). As observed in the immunofluorescence study, fractionation studies confirmed that NC protein could be localized to both the nucleus and nucleolus and the same was observed even LMB treatment, an inhibitor of CRM1-dependent nuclear export (Fig. 1B). Furthermore, the subcellular distribution pattern of NC-EGFP also showed localization in cytoplasm, nucleus and nucleolus as untagged NC protein (Fig. 1C). To study further NC localization in the nucleus in more detail, we co-stained with LMNA, a nuclear membrane marker protein, and B23, a nucleolar marker protein (McKeon et al., 1986 and Spector et al., 1984), and found that NC co-localized with both the nuclear membrane and nucleolus markers (Fig. 1D and E)

Figure 1A-E||complete
CACAO 11399

on HV1H2:GAG
Flagged
Private
Assessment		Amcmillan2016-04-05 20:18:17 CDTYou need to be an instructor to view these notes.Requires Changes
✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
✔ Evidence
✔ With/From
✗ Notes
✔ Unique/Original


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