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Cacao

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GO:0030255protein secretion by the type IV secretion systemPMID:23950720IDA: Inferred from Direct Assay P
This annotation made on page: BRUA2:Q2YN43
By: Annie luca (group Team You cannot B. cereus) on 2015-10-04 22:01:11 CDT.




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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
Private
Assessment
Suzialeksander2015-12-08 16:54:36 CSTYou need to be an instructor to view these notes.Updated by Instructor
Private
Assessment
JimHu2015-12-01 13:54:39 CSTYou need to be an instructor to view these notes.Requires Changes
Protein
Publication
Qualifier
Go term
Evidence
With/From
Unique/Original
Public
Assessment
DanielRenfro2015-11-17 13:20:09 CST

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0052208 modification of morphology or physiology of other organism via substance secreted by type IV secretion system involved in symbiotic interaction PMID:23950720 IDA: Inferred from Direct Assay P Figure 1. TEM1 b-lactamase protein translocation reporter assay. Shows translocation of effector protein BspB (BAB1_0712)

Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells. Shows effector protein BspB (BAB1_0712) translocated by T4SS.

Figure 4. A subset of Bsp effectors target the host secretory pathway. Shows that effector protein BspB (BAB1_0712) targets secretory pathway by colocalization with calnexin a marker of ER.

Figure 5. Ectopic expression of BspB (BAB1_0712) in HeLa cells interferes with the host secretory pathway.

Figure 7. Contribution of BspB (BAB1_0712) to inhibition of host cell secretion during infection.||complete
CACAO 10997

on BRUA2:Q2YN43
Flagged
Public
Assessment
DanielRenfro2015-11-10 19:53:36 CST

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0052050 translocation of peptides or proteins into host PMID:23950720 IDA: Inferred from Direct Assay P Figure 1. TEM1 b-lactamase protein translocation reporter assay.

Panel A and B shows percentage of shift in fluorescence emission that correlates with protein Brucella putative effector BAB1_0712 being translocated into J774A.1 host cell (murine cell line). Panel C and D shows representative fluorescence micrographs of protein translocation. Also look Figure S1 and S2.

Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells. Panel A. B. abortus strain 2308 (black bars) or virB9 mutant strain (white bars) expressing the CyaA fusions to the indicated Brucella effector proteins were used to infect J774.A1 cells, and the cAMP level of infected cells was determined.||complete
CACAO 10997

on BRUA2:Q2YN43
Flagged
Private
Assessment
JimHu2015-11-03 20:27:10 CSTYou need to be an instructor to view these notes.Requires Changes
Go term
Notes
Public
Assessment
DanielRenfro2015-11-03 15:48:26 CST

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0042000 translocation of peptides or proteins into host PMID:23950720 IDA: Inferred from Direct Assay P Figure 1. TEM1 b-lactamase protein translocation reporter assay.

Panel A and B shows percentage of shift in fluorescence emission that correlates with protein Brucella putative effector BAB1_0712 being translocated into J774A.1 host cell (murine cell line). Panel C and D shows representative fluorescence micrographs of protein translocation. Also look Figure S1 and S2.

Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells. Panel A. B. abortus strain 2308 (black bars) or virB9 mutant strain (white bars) expressing the CyaA fusions to the indicated Brucella effector proteins were used to infect J774.A1 cells, and the cAMP level of infected cells was determined.||complete
CACAO 10997

on BRUA2:Q2YN43
Flagged
Private
Assessment
Suzialeksander2015-10-26 15:28:50 CDTYou need to be an instructor to view these notes.Requires Changes
Go term
Evidence
Notes
Public
Assessment
DanielRenfro2015-10-18 18:11:40 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0044097 protein secretion by the type IV secretion system PMID:23950720 IDA: Inferred from Direct Assay P Figure 1. TEM1 b-lactamase protein translocation reporter assay.

Panel A and B shows percentage of shift in fluorescence emission that correlates with protein Brucella putative effector BAB1_0712 being translocated into J774A.1 host cell (murine cell line). Panel C and D shows representative fluorescence micrographs of protein translocation. Also look Figure S1 and S2.

Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells. Panel A. B. abortus strain 2308 (black bars) or virB9 mutant strain (white bars) expressing the CyaA fusions to the indicated Brucella effector proteins were used to infect J774.A1 cells, and the cAMP level of infected cells was determined.||complete
CACAO 10997

on BRUA2:Q2YN43
Flagged
Private
Assessment
Suzialeksander2015-10-13 18:15:26 CDTYou need to be an instructor to view these notes.Requires Changes
Protein
Publication
Qualifier
Evidence
With/From
Notes
Unique/Original