Private Assessment | Suzialeksander | 2015-12-08 16:54:36 CST | You need to be an instructor to view these notes. | Updated by Instructor
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Private Assessment | JimHu | 2015-12-01 13:54:39 CST | You need to be an instructor to view these notes. | Requires Changes
✔ Protein ✔ Publication ✔ Qualifier ✗ Go term ✔ Evidence ✔ With/From ✔ Unique/Original
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Public Assessment | DanielRenfro | 2015-11-17 13:20:09 CST | This annotation has been flagged because it has been edited since last assessment
Qualifier |
GO ID |
GO term name |
Reference |
Evidence Code |
with/from |
Aspect |
Notes |
Status
|
|
GO:0052208 |
modification of morphology or physiology of other organism via substance secreted by type IV secretion system involved in symbiotic interaction |
PMID:23950720 |
IDA: Inferred from Direct Assay |
|
P |
Figure 1. TEM1 b-lactamase protein translocation reporter assay. Shows translocation of effector protein BspB (BAB1_0712)
Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells. Shows effector protein BspB (BAB1_0712) translocated by T4SS.
Figure 4. A subset of Bsp effectors target the host secretory pathway. Shows that effector protein BspB (BAB1_0712) targets secretory pathway by colocalization with calnexin a marker of ER.
Figure 5. Ectopic expression of BspB (BAB1_0712) in HeLa cells interferes with the host secretory pathway.
Figure 7. Contribution of BspB (BAB1_0712) to inhibition of host cell secretion during infection.||complete CACAO 10997
| on BRUA2:Q2YN43
| Flagged
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Public Assessment | DanielRenfro | 2015-11-10 19:53:36 CST | This annotation has been flagged because it has been edited since last assessment
Qualifier |
GO ID |
GO term name |
Reference |
Evidence Code |
with/from |
Aspect |
Notes |
Status
|
|
GO:0052050 |
translocation of peptides or proteins into host |
PMID:23950720 |
IDA: Inferred from Direct Assay |
|
P |
Figure 1. TEM1 b-lactamase protein translocation reporter assay.
Panel A and B shows percentage of shift in fluorescence emission that correlates with protein Brucella putative effector BAB1_0712 being translocated into J774A.1 host cell (murine cell line).
Panel C and D shows representative fluorescence micrographs of protein translocation. Also look Figure S1 and S2.
Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells.
Panel A. B. abortus strain 2308 (black bars) or virB9 mutant strain (white bars) expressing the CyaA fusions to the indicated Brucella effector proteins were used to infect J774.A1 cells, and the cAMP level of infected cells was determined.||complete CACAO 10997
| on BRUA2:Q2YN43
| Flagged
|
Private Assessment | JimHu | 2015-11-03 20:27:10 CST | You need to be an instructor to view these notes. | Requires Changes
✗ Go term ✗ Notes
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Public Assessment | DanielRenfro | 2015-11-03 15:48:26 CST | This annotation has been flagged because it has been edited since last assessment
Qualifier |
GO ID |
GO term name |
Reference |
Evidence Code |
with/from |
Aspect |
Notes |
Status
|
|
GO:0042000 |
translocation of peptides or proteins into host |
PMID:23950720 |
IDA: Inferred from Direct Assay |
|
P |
Figure 1. TEM1 b-lactamase protein translocation reporter assay.
Panel A and B shows percentage of shift in fluorescence emission that correlates with protein Brucella putative effector BAB1_0712 being translocated into J774A.1 host cell (murine cell line).
Panel C and D shows representative fluorescence micrographs of protein translocation. Also look Figure S1 and S2.
Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells.
Panel A. B. abortus strain 2308 (black bars) or virB9 mutant strain (white bars) expressing the CyaA fusions to the indicated Brucella effector proteins were used to infect J774.A1 cells, and the cAMP level of infected cells was determined.||complete CACAO 10997
| on BRUA2:Q2YN43
| Flagged
|
Private Assessment | Suzialeksander | 2015-10-26 15:28:50 CDT | You need to be an instructor to view these notes. | Requires Changes
✗ Go term ✗ Evidence ✗ Notes
|
Public Assessment | DanielRenfro | 2015-10-18 18:11:40 CDT | This annotation has been flagged because it has been edited since last assessment
Qualifier |
GO ID |
GO term name |
Reference |
Evidence Code |
with/from |
Aspect |
Notes |
Status
|
|
GO:0044097 |
protein secretion by the type IV secretion system |
PMID:23950720 |
IDA: Inferred from Direct Assay |
|
P |
Figure 1. TEM1 b-lactamase protein translocation reporter assay.
Panel A and B shows percentage of shift in fluorescence emission that correlates with protein Brucella putative effector BAB1_0712 being translocated into J774A.1 host cell (murine cell line).
Panel C and D shows representative fluorescence micrographs of protein translocation. Also look Figure S1 and S2.
Figure 2. VirB-dependent translocation of Brucella BsP effectors into J774.A1 cells.
Panel A. B. abortus strain 2308 (black bars) or virB9 mutant strain (white bars) expressing the CyaA fusions to the indicated Brucella effector proteins were used to infect J774.A1 cells, and the cAMP level of infected cells was determined.||complete CACAO 10997
| on BRUA2:Q2YN43
| Flagged
|
Private Assessment | Suzialeksander | 2015-10-13 18:15:26 CDT | You need to be an instructor to view these notes. | Requires Changes
✔ Protein ✔ Publication ✔ Qualifier ✗ Evidence ✔ With/From ✗ Notes ✔ Unique/Original
|