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Cacao

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GO:0004527ISA: Inferred from Sequence Alignment UniProtKB:G4FG24 UniProtKB:S5YPI2
This annotation made on page: 9CAUD:S5YPI2
By: Njulie (group TeamBlue) on 2015-04-08 08:55:51 CDT.



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Entry TypeChallenging User,GroupTime/DateChallenge ReasonPoints/Assessment
ChallengeYravali,
TeamGreen		2015-04-13 10:29:40 CDT

GO term is missing and in the notes section if more information regarding the gene function and the experiment done is provided, it would be better.

None, yet.
ChallengeHasli,
TeamPurple		2015-04-13 10:14:17 CDT

What is the GO term and the reference for this annotation?

None, yet.
Private
Assessment		Ivanerill2015-05-12 14:30:52 CDTYou need to be an instructor to view these notes.Acceptable
✔ Protein
✔ Publication
✔ Qualifier
✔ Go term
✔ Evidence
✔ With/From
✔ Notes
✔ Unique/Original
Public
Assessment		DanielRenfro2015-05-11 16:30:56 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003677 exonuclease activity GO_REF:0000100 ISA: Inferred from Sequence Alignment UniProtKB:Q9X1X0 F An HHPred job of S5YPI2 provides evidence that G4FG24 and S5YPI2 have sequence alignment.

BLASTP: BLAST YP_008430900.1 (S5YPI2) YP_007977993 (Q9X1X0) is a result with E-value of 2e-10, score of 70.9, query coverage of 91%, 26% identity.

HHPred: The protein sequence of S5YPI2 returns the first result 3THO which is part of Q9X1X0 (Score: 296.59; E-value: 2e-38; Similarity: 0.301; % Identity: 23)

Protein may be dual exonuclease/metallophosphatase with DNA-binding activity.||complete
CACAO 10893

on 9CAUD:S5YPI2
Flagged
Public
Assessment		DanielRenfro2015-05-11 14:05:16 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0004527 exonuclease activity GO_REF:0000100 ISA: Inferred from Sequence Alignment UniProtKB:Q9X1X0 F An HHPred job of S5YPI2 provides evidence that G4FG24 and S5YPI2 have sequence alignment.

BLASTP: BLAST YP_008430900.1 (S5YPI2) YP_007977993 (Q9X1X0) is a result with E-value of 2e-10, score of 70.9, query coverage of 91%, 26% identity.

HHPred: The protein sequence of S5YPI2 returns the first result 3THO which is part of Q9X1X0 (Score: 296.59; E-value: 2e-38; Similarity: 0.301; % Identity: 23)

Protein may be dual exonuclease/metallophosphatase with DNA-binding activity.||complete
CACAO 10893

on 9CAUD:S5YPI2
Flagged
Public
Assessment		DanielRenfro2015-05-11 14:02:04 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0004527 exonuclease activity PMID:21937514 ISA: Inferred from Sequence Alignment UniProtKB:Q9X1X0 F An HHPred job of S5YPI2 provides evidence that G4FG24 and S5YPI2 have sequence alignment.

BLASTP: BLAST YP_008430900.1 (S5YPI2) YP_007977993 (Q9X1X0) is a result with E-value of 2e-10, score of 70.9, query coverage of 91%, 26% identity.

HHPred: The protein sequence of S5YPI2 returns the first result 3THO which is part of Q9X1X0 (Score: 296.59; E-value: 2e-38; Similarity: 0.301; % Identity: 23)

Protein may be dual exonuclease/metallophosphatase with DNA-binding activity.||complete
CACAO 10893

on 9CAUD:S5YPI2
Flagged
Public
Assessment		DanielRenfro2015-04-21 21:51:53 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0004527 exonuclease activity PMID:21937514 ISA: Inferred from Sequence Alignment UniProtKB:G4FG24 F A BLASTP and HHPred job of S5YPI2 (http://www.ncbi.nlm.nih.gov/protein/YP_008430900.1) provides evidence that G4FG24 (http://www.ncbi.nlm.nih.gov/protein/YP_007977993.1?report=genpept) S5YPI2 has sequence alignment with G4FG24 based on BLAST results and HHPred.

BLASTP: BLAST YP_008430900.1 excluding eukarya for 5000 results. YP_007977993 is a result with E-value of 2e-10, score of 70.9, query coverage of 91%, 26% identity.

HHPred: The protein sequence of S5YPI2 returns the first result 3THO http://www.rcsb.org/pdb/explore.do?structureId=3THO which is a putative exonuclease of Thermotoga maritima Score: 296.59 E-value: 2e-38 Similarity: 0.301 % Identity: 23

Protein may be dual exonuclease/metallophosphatase (see http://www.ncbi.nlm.nih.gov/protein/YP_007977993.1?report=genpept)%7C%7Ccomplete
CACAO 10893

on 9CAUD:S5YPI2
Flagged
Public
Assessment		DanielRenfro2015-04-21 21:20:32 CDT

This annotation has been flagged because it has been edited since last assessment

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0004527 exonuclease activity PMID:21937514 ISA: Inferred from Sequence Alignment UniProtKB:G4FG24 F A BLASTP and HHPred job of G4FG24 (http://www.ncbi.nlm.nih.gov/protein/YP_007977993.1?report=genpept) provides evidence that S5YPI2 (http://www.ncbi.nlm.nih.gov/protein/YP_008430900.1) has sequence alignment with G4FG24 based on BLAST results and HHPred.

E-value=2.3e-38 Score=296.58 Identities=23% Similarity=0.301||complete
CACAO 10893

on 9CAUD:S5YPI2
Flagged
Public
Assessment		Ivanerill2015-04-21 17:35:33 CDT

You should cite the GO_REF for a transfer annotation. What are the details (e-value and such) in the notes for? BLASTP? Don't cite the own gene in the WITH field. Is there a valid GO annotation for UniProtKB:G4FG24 with that GO term?

Requires Changes
✗ Publication
✔ Qualifier
✗ Go term
✗ Evidence
✗ With/From
✗ Notes
✔ Unique/Original


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