GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
Cacao
| GO:0042597 | periplasmic space | PMID:20734329 | IDA: Inferred from Direct Assay | C | ||||
| This annotation made on page: LAMBD:SPAN1 By: JPShaw (group Team RNA) on 2014-11-23 16:30:30 CST. | ||||||||
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| Entry Type | Challenging User,Group | Time/Date | Challenge Reason | Points/Assessment |
|---|---|---|---|---|
| Challenge | JPShaw, Team RNA | 2014-11-24 01:18:54 CST |
| None, yet. |
| Challenge | JPShaw, Team RNA | 2014-11-24 01:04:22 CST | DEFENSE: figure 7 only shows the dimensions of the periplasmic space, and does show protein localization in this domain. "Determination of periplasmic dimensions using cryo-electron microscopy. E. coli K-12 cells in logarithmic phase were plunged into liquid ethane and imaged by cryo-electron microscopy. Shown is a representative whole, frozen-hydrated cell with an inset box indicating the region of interest. The average distance between the inner and outer membranes at the mid-length of 31 such cells was 25.5 ± 3.1 nm. The periplasmic distance increases at the poles as shown in this example" While a GO-term describing protein localization to the outer membrane is fitting to this protein, this paper shows it has a domain in the periplasm...which is what the annotation is describing. A second annotation from a separate paper would be needed to properly demonstrate and annotate protein localization to the outer membrane. | None, yet. |
| Challenge | Roosheel, Team Thoth | 2014-11-23 19:11:21 CST | Figure 2 shows the purification of the proteins. The actual periplasmic localization of the Rz and Rz1 domains is seen in figure 7. A better GO term would be: 0089705: Protien localization to outer membrane | 0 |
| Public Assessment | JimHu | 2014-12-02 13:20:27 CST | This paper shows interaction of Rz and Rz1 which cannot be annotated with GO terms allowed in CACAO. Periplasmic localization was shown in other work. | Unacceptable ✗ Publication ✗ Notes |
