RHOMR:O33897

Species (Taxon ID)	Rhodothermus marinus (Rhodothermus obamensis). (29549)
Gene Name(s)	celA (ECO:0000313 with EMBL:AAB65594.1)
Protein Name(s)	Cellulase (ECO:0000313 with EMBL:AAB65594.1)
External Links
UniProt	O33897
EMBL	U72637
PDB	1H0B 2BW8 2BWA 2BWC 3B7M
PDBsum	1H0B 2BW8 2BWA 2BWC 3B7M
ProteinModelPortal	O33897
SMR	O33897
CAZy	GH12
EvolutionaryTrace	O33897
GO	GO:0008810 GO:0000272
Gene3D	2.60.120.180
InterPro	IPR013320 IPR013319 IPR002594
Pfam	PF01670
SUPFAM	SSF49899

Annotations

Qualifier	GO ID	GO term name	Reference	ECO ID	ECO term name	with/from	Aspect	Extension	Notes	Status
	GO:0008810	cellulase activity	PMID:9581291	ECO:0000314			F		Figures 2,3, and 4 show SDS page gels for the native CelA enzyme, the recombinant form of the enzyme, and a gel comparing the activity and protein of both. Figure 2 shows the native CelA molecular mass corresponds to the expected value. This information is important for future comparisons to recombinant types. Figure 3 shows the recombinant CelA molecular mass and activity after boiling with proteinase K. The control (lane 2) showed activity bands at higher molecular weights than the native enzyme. After boiling, activity began to disappear and a protein band of 26 kDa appeared in lanes 3, 4, and 5. Figure 4 shows an SDS gel with CMC for both enzymes. The native activity band appeared in a corresponding position to the smallest activity band of the recombinant enzyme. The same activity bands were seen regardless of heating with protease.	complete CACAO 4434
enables	GO:0008810	cellulase activity	PMID:9581291	ECO:0000314	direct assay evidence used in manual assertion		F		Seeded From UniProt	complete
involved_in	GO:0000272	polysaccharide catabolic process	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR002594	P		Seeded From UniProt	complete
enables	GO:0004553	hydrolase activity, hydrolyzing O-glycosyl compounds	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR013319	F		Seeded From UniProt	complete
enables	GO:0008810	cellulase activity	GO_REF:0000002	ECO:0000256	match to sequence model evidence used in automatic assertion	InterPro:IPR002594	F		Seeded From UniProt	complete
enables	GO:0008810	cellulase activity	GO_REF:0000003	ECO:0000501	evidence used in automatic assertion	EC:3.2.1.4	F		Seeded From UniProt	complete
enables	GO:0016787	hydrolase activity	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0378	F		Seeded From UniProt	complete
enables	GO:0016798	hydrolase activity, acting on glycosyl bonds	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0326	F		Seeded From UniProt	complete
involved_in	GO:0008152	metabolic process	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0326	P		Seeded From UniProt	complete
involved_in	GO:0000272	polysaccharide catabolic process	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0624	P		Seeded From UniProt	complete
involved_in	GO:0005975	carbohydrate metabolic process	GO_REF:0000038	ECO:0000323	imported automatically asserted information used in automatic assertion	UniProtKB-KW:KW-0119	P		Seeded From UniProt	complete
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References

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