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PSEAI:Q51353

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Species (Taxon ID) Pseudomonas aeruginosa. (287)
Gene Name(s) pilO (ECO:0000313 with EMBL:AAA87404.1)
Protein Name(s) PilO protein (ECO:0000313 with EMBL:AAA87404.1)

Pilus assembly , PilO family protein (ECO:0000313 with EMBL:KFL09795.1) Pilus assembly protein PilP (ECO:0000313 with EMBL:KDR46614.1) Type 4 fimbrial biogenesis protein PilO (ECO:0000313 with EMBL:CDO84111.1)

External Links
UniProt Q51353
EMBL U12892
HG974234
JJOD01000077
JHEU01000024
JMQH01000012
JFJU01000004
JOVK01000001
JPQQ01000013
JMBS01000005
PIR S77728
RefSeq NP_253729.1
PDB 2RJZ
PDBsum 2RJZ
SMR Q51353
STRING 208964.PA5042
PRIDE Q51353
DNASU 880985
GeneID 880985
KEGG pae:PA5042
PATRIC 19844915
PseudoCAP PA5042
HOGENOM HOG000281383
KO K02664
OrthoDB EOG6VQPV4
EvolutionaryTrace Q51353
GO GO:0004576
GO:0008152
GO:0043683
GO:0043107
Gene3D 1.10.287.540
3.30.70.60
InterPro IPR007445
IPR014717
IPR023218
Pfam PF04350
PIRSF PIRSF016482

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0004576

oligosaccharyl transferase activity

PMID:17890310[1]

ECO:0000314

F

Figure 1a shows that the pilO knockout mutant is incapable of producing glycosylated pillin, whereas the wild-type strain did produce glycosylated pillin. Figure 1a shows the picture of the gel from western-blot analysis of whole cell extracts from both the wild-type strain and the pilO mutatnt containing unglycosylated and glycosylated pillin.

complete
CACAO 8050

enables

GO:0004576

oligosaccharyl transferase activity

PMID:17890310[1]

ECO:0000314

direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

part_of

GO:0044096

type IV pilus

PMID:7565110[2]

ECO:0000315

mutant phenotype evidence used in manual assertion

C

Seeded From UniProt

complete

involved_in

GO:0043683

type IV pilus biogenesis

PMID:7565110[2]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0043683

type IV pilus biogenesis

PMID:23457250[3]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0043683

type IV pilus biogenesis

PMID:22053789[4]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0043683

type IV pilus biogenesis

PMID:19857645[5]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0043107

type IV pilus-dependent motility

PMID:23457250[3]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0043107

type IV pilus-dependent motility

PMID:22053789[4]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0043107

type IV pilus-dependent motility

PMID:19857645[5]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

part_of

GO:0016020

membrane

GO_REF:0000038

ECO:0000323

imported automatically asserted information used in automatic assertion

UniProtKB-KW:KW-0472

C

Seeded From UniProt

complete

part_of

GO:0016021

integral component of membrane

GO_REF:0000038

ECO:0000323

imported automatically asserted information used in automatic assertion

UniProtKB-KW:KW-0812

C

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 Faridmoayer, A et al. (2007) Functional characterization of bacterial oligosaccharyltransferases involved in O-linked protein glycosylation. J. Bacteriol. 189 8088-98 PubMed GONUTS page
  2. 2.0 2.1 Martin, PR et al. (1995) Characterization of a five-gene cluster required for the biogenesis of type 4 fimbriae in Pseudomonas aeruginosa. Mol. Microbiol. 16 497-508 PubMed GONUTS page
  3. 3.0 3.1 Tammam, S et al. (2013) PilMNOPQ from the Pseudomonas aeruginosa type IV pilus system form a transenvelope protein interaction network that interacts with PilA. J. Bacteriol. 195 2126-35 PubMed GONUTS page
  4. 4.0 4.1 Tammam, S et al. (2011) Characterization of the PilN, PilO and PilP type IVa pilus subcomplex. Mol. Microbiol. 82 1496-514 PubMed GONUTS page
  5. 5.0 5.1 Ayers, M et al. (2009) PilM/N/O/P proteins form an inner membrane complex that affects the stability of the Pseudomonas aeruginosa type IV pilus secretin. J. Mol. Biol. 394 128-42 PubMed GONUTS page