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PSEAE:RHLR

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Species (Taxon ID) Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG12228). (208964)
Gene Name(s) rhlR (synonyms: lasM, vsmR)
Protein Name(s) Regulatory protein RhlR

Elastase modulator

External Links
UniProt P54292
EMBL U40458
L08962
U15644
AE004091
L28170
PIR B83212
C53652
S70174
RefSeq NP_252167.1
ProteinModelPortal P54292
SMR P54292
STRING 208964.PA3477
ChEMBL CHEMBL3112386
EnsemblBacteria AAG06865
GeneID 878968
KEGG pae:PA3477
PATRIC 19841565
PseudoCAP PA3477
eggNOG COG2771
HOGENOM HOG000111053
InParanoid P54292
KO K18099
OMA VMPPEMK
OrthoDB EOG6P8TQG
PhylomeDB P54292
Proteomes UP000002438
GO GO:0003677
GO:0003700
GO:0010467
GO:0046889
GO:0045862
GO:1900378
GO:0009372
GO:0006351
Gene3D 1.10.10.10
3.30.450.80
InterPro IPR016032
IPR005143
IPR000792
IPR011991
Pfam PF03472
PF00196
PRINTS PR00038
SMART SM00421
SUPFAM SSF46894
SSF75516
PROSITE PS00622
PS50043

Annotations

Qualifier GO ID GO term name Reference ECO ID ECO term name with/from Aspect Extension Notes Status
GO:0006355

regulation of transcription, DNA-dependent

PMID:8522523[1]

ECO:0000315

P

Table 3 shows that cells containing deficient rhlR significantly decrease the transcription of lasB compared to cells containing a fully functional rhlR. Showing that rhlR is a regulator of transcription.

complete

GO:0009372

quorum sensing

PMID:9150205[2]

ECO:0000314

P

Figure 1A and B show that rhlR is mroe strongly induced when in the presence of autoinducers which shows that rhlR is involved in quorum sensing of P. aeruginosa.

complete

involved_in

GO:0010467

gene expression

PMID:9294432[3]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

GO:0010467

gene expression

PMID:9294432[3]

ECO:0000315

P

Figure 4A shows that RhlR was needed for the expression of rhlA9-lacZ in E. coli DH5a(pECP61.5)

complete
CACAO 5026

involved_in

GO:0045893

positive regulation of transcription, DNA-templated

PMID:11053384[4]

ECO:0005648

luciferase reporter gene assay evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0045893

positive regulation of transcription, DNA-templated

PMID:11359576[5]

ECO:0000288

RNA protection assay evidence used in manual assertion

P

Seeded From UniProt

complete

part_of

GO:0032993

protein-DNA complex

PMID:24935161[6]

ECO:0005667

site-directed mutagenesis evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0032993

protein-DNA complex

PMID:22262098[7]

ECO:0005667

site-directed mutagenesis evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0032993

protein-DNA complex

PMID:16735731[8]

ECO:0005667

site-directed mutagenesis evidence used in manual assertion

C

Seeded From UniProt

complete

part_of

GO:0032993

protein-DNA complex

PMID:14526008[9]

ECO:0001807

electrophoretic mobility shift assay evidence used in manual assertion

C

Seeded From UniProt

complete

enables

GO:0001217

DNA-binding transcription repressor activity

PMID:16735731[8]

ECO:0005667

site-directed mutagenesis evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0001217

DNA-binding transcription repressor activity

PMID:14526008[9]

ECO:0001807

electrophoretic mobility shift assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0001216

DNA-binding transcription activator activity

PMID:24935161[6]

ECO:0005667

site-directed mutagenesis evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0001216

DNA-binding transcription activator activity

PMID:22262098[7]

ECO:0005667

site-directed mutagenesis evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0001216

DNA-binding transcription activator activity

PMID:14526008[9]

ECO:0001807

electrophoretic mobility shift assay evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0000976

transcription regulatory region sequence-specific DNA binding

PMID:24935161[6]

ECO:0005667

site-directed mutagenesis evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0000976

transcription regulatory region sequence-specific DNA binding

PMID:22262098[7]

ECO:0005667

site-directed mutagenesis evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0000976

transcription regulatory region sequence-specific DNA binding

PMID:16735731[8]

ECO:0005667

site-directed mutagenesis evidence used in manual assertion

F

Seeded From UniProt

complete

enables

GO:0000976

transcription regulatory region sequence-specific DNA binding

PMID:14526008[9]

ECO:0001807

electrophoretic mobility shift assay evidence used in manual assertion

F

Seeded From UniProt

complete

involved_in

GO:1900378

positive regulation of secondary metabolite biosynthetic process

PMID:8522523[1]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0046889

positive regulation of lipid biosynthetic process

PMID:8144472[10]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0045862

positive regulation of proteolysis

PMID:8522523[1]

ECO:0000315

mutant phenotype evidence used in manual assertion

P

Seeded From UniProt

complete

involved_in

GO:0045892

negative regulation of transcription, DNA-templated

GO_REF:0000108

ECO:0000364

evidence based on logical inference from manual annotation used in automatic assertion

GO:0001217

P

Seeded From UniProt

complete

involved_in

GO:0045892

negative regulation of transcription, DNA-templated

GO_REF:0000108

ECO:0000364

evidence based on logical inference from manual annotation used in automatic assertion

GO:0001217

P

Seeded From UniProt

complete

enables

GO:0003677

DNA binding

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR016032

F

Seeded From UniProt

complete

involved_in

GO:0006355

regulation of transcription, DNA-templated

GO_REF:0000002

ECO:0000256

match to sequence model evidence used in automatic assertion

InterPro:IPR000792
InterPro:IPR016032

P

Seeded From UniProt

complete

enables

GO:0003677

DNA binding

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0238

F

Seeded From UniProt

complete

involved_in

GO:0009372

quorum sensing

GO_REF:0000037

ECO:0000322

imported manually asserted information used in automatic assertion

UniProtKB-KW:KW-0673

P

Seeded From UniProt

complete

Notes

References

See Help:References for how to manage references in GONUTS.

  1. 1.0 1.1 1.2 Brint, JM & Ohman, DE (1995) Synthesis of multiple exoproducts in Pseudomonas aeruginosa is under the control of RhlR-RhlI, another set of regulators in strain PAO1 with homology to the autoinducer-responsive LuxR-LuxI family. J. Bacteriol. 177 7155-63 PubMed GONUTS page
  2. Pesci, EC et al. (1997) Regulation of las and rhl quorum sensing in Pseudomonas aeruginosa. J. Bacteriol. 179 3127-32 PubMed GONUTS page
  3. 3.0 3.1 Pearson, JP et al. (1997) Roles of Pseudomonas aeruginosa las and rhl quorum-sensing systems in control of elastase and rhamnolipid biosynthesis genes. J. Bacteriol. 179 5756-67 PubMed GONUTS page
  4. Winzer, K et al. (2000) The Pseudomonas aeruginosa lectins PA-IL and PA-IIL are controlled by quorum sensing and by RpoS. J. Bacteriol. 182 6401-11 PubMed GONUTS page
  5. Rahim, R et al. (2001) Cloning and functional characterization of the Pseudomonas aeruginosa rhlC gene that encodes rhamnosyltransferase 2, an enzyme responsible for di-rhamnolipid biosynthesis. Mol. Microbiol. 40 708-18 PubMed GONUTS page
  6. 6.0 6.1 6.2 González-Valdez, A et al. (2014) The effect of specific rhlA-las-box mutations on DNA binding and gene activation by Pseudomonas aeruginosa quorum-sensing transcriptional regulators RhlR and LasR. FEMS Microbiol. Lett. 356 217-25 PubMed GONUTS page
  7. 7.0 7.1 7.2 Aguirre-Ramírez, M et al. (2012) The Pseudomonas aeruginosa rmlBDAC operon, encoding dTDP-L-rhamnose biosynthetic enzymes, is regulated by the quorum-sensing transcriptional regulator RhlR and the alternative sigma factor σS. Microbiology (Reading, Engl.) 158 908-16 PubMed GONUTS page
  8. 8.0 8.1 8.2 Xiao, G et al. (2006) Mutation analysis of the Pseudomonas aeruginosa mvfR and pqsABCDE gene promoters demonstrates complex quorum-sensing circuitry. Microbiology (Reading, Engl.) 152 1679-86 PubMed GONUTS page
  9. 9.0 9.1 9.2 9.3 Medina, G et al. (2003) Mechanism of Pseudomonas aeruginosa RhlR transcriptional regulation of the rhlAB promoter. J. Bacteriol. 185 5976-83 PubMed GONUTS page
  10. Ochsner, UA et al. (1994) Isolation and characterization of a regulatory gene affecting rhamnolipid biosurfactant synthesis in Pseudomonas aeruginosa. J. Bacteriol. 176 2044-54 PubMed GONUTS page