PMID:9990856

Citation	Tóth, A, Ciosk, R, Uhlmann, F, Galova, M, Schleiffer, A and Nasmyth, K (1999) Yeast cohesin complex requires a conserved protein, Eco1p(Ctf7), to establish cohesion between sister chromatids during DNA replication. Genes Dev. 13:320-33
Abstract	Sister chromatid cohesion is crucial for chromosome segregation during mitosis. Loss of cohesion very possibly triggers sister separation at the metaphase --> anaphase transition. This process depends on the destruction of anaphase inhibitory proteins like Pds1p (Cut2p), which is thought to liberate a sister-separating protein Esp1p (Cut1p). By looking for mutants that separate sister centromeres in the presence of Pds1p, this and a previous study have identified six proteins essential for establishing or maintaining sister chromatid cohesion. Four of these proteins, Scc1p, Scc3p, Smc1p, and Smc3p, are subunits of a 'Cohesin' complex that binds chromosomes from late G1 until the onset of anaphase. The fifth protein, Scc2p, is not a stoichiometric Cohesin subunit but it is required for Cohesin's association with chromosomes. The sixth protein, Eco1p(Ctf7p), is not a Cohesin subunit. It is necessary for the establishment of cohesion during DNA replication but not for its maintenance during G2 and M phases.
Links	PubMed PMC316435
Keywords	Amino Acid Sequence; Animals; Cell Cycle Proteins/metabolism; Chromatin; Chromosomal Proteins, Non-Histone; Conserved Sequence; DNA Replication; Fungal Proteins/genetics; Fungal Proteins/metabolism; Fungal Proteins/physiology; Genes, Fungal; Mice; Molecular Sequence Data; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Nuclear Proteins/physiology; Phosphoproteins; S Phase; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid
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