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PMID:9921650
| Citation |
Roelen, BA, Goumans, MJ, Zwijsen, A and Mummery, CL (1998) Identification of two distinct functions for TGF-beta in early mouse development. Differentiation 64:19-31 |
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| Abstract |
In this study the function of transforming growth factor-beta (TGF-beta) in preimplantation mouse embryos was examined. By RT-PCR, mRNA for the signalling type I (T beta R-I) and type II (T beta R-II) receptors for TGF-beta was shown to be present in two distinct time windows: in fertilized oocytes and at the blastocyst stage. The function of TGF-beta at these times was analysed in two ways. Firstly, the TGF-beta signalling pathway was blocked by injecting a DNA construct encoding a truncated T beta R-II, that acts as a dominant-negative receptor, in fertilized oocytes, and the effect on development was determined. Secondly, inner cell masses isolated at the blastocyst stage were cultured in vitro with and without TGF-beta under conditions that favour the outgrowth of parietal endoderm. The results show that TGF-beta signalling mediated by maternally expressed receptors is important for development of preimplantation embryos beyond the two-cell stage, and suggest a regulatory role for TGF-beta in the outgrowth of parietal endoderm. |
| Links |
PubMed Online version:10.1046/j.1432-0436.1998.6410019.x |
| Keywords |
Activin Receptors, Type I; Animals; Blastocyst/metabolism; Embryonic and Fetal Development/physiology; Endoderm/drug effects; Endoderm/physiology; Female; Fibronectins/antagonists & inhibitors; Fibronectins/pharmacology; Male; Mice; Mice, Inbred C57BL; Mice, Inbred CBA; Microinjections; Organ Culture Techniques; Protein-Serine-Threonine Kinases/biosynthesis; Protein-Serine-Threonine Kinases/genetics; RNA, Messenger/analysis; Receptors, Transforming Growth Factor beta/biosynthesis; Receptors, Transforming Growth Factor beta/genetics; Sequence Deletion; Signal Transduction/drug effects; Transforming Growth Factor beta/pharmacology; Transforming Growth Factor beta/physiology; Zygote/metabolism |
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