GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:9864365
| Citation |
Pruyne, DW, Schott, DH and Bretscher, A (1998) Tropomyosin-containing actin cables direct the Myo2p-dependent polarized delivery of secretory vesicles in budding yeast. J. Cell Biol. 143:1931-45 |
|---|---|
| Abstract |
The actin cytoskeleton in budding yeast consists of cortical patches and cables, both of which polarize toward regions of cell growth. Tropomyosin localizes specifically to actin cables and not cortical patches. Upon shifting cells with conditionally defective tropomyosin to restrictive temperatures, actin cables disappear within 1 min and both the unconventional class V myosin Myo2p and the secretory vesicle-associated Rab GTPase Sec4p depolarize rapidly. Bud growth ceases and the mother cell grows isotropically. When returned to permissive temperatures, tropomyosin-containing cables reform within 1 min in polarized arrays. Cable reassembly permits rapid enrichment of Myo2p at the focus of nascent cables as well as the Myo2p- dependent recruitment of Sec4p and the exocyst protein Sec8p, and the initiation of bud emergence. With the loss of actin cables, cortical patches slowly assume an isotropic distribution within the cell and will repolarize only after restoration of cables. Therefore, actin cables respond to polarity cues independently of the overall distribution of cortical patches and are able to directly target the Myo2p-dependent delivery of secretory vesicles and polarization of growth. |
| Links | |
| Keywords |
Actin Cytoskeleton/physiology; Actins/physiology; Carrier Proteins/physiology; Cell Polarity; Exocytosis/physiology; Fungal Proteins/physiology; GTP-Binding Proteins/physiology; Myosin Heavy Chains; Myosin Type II; Myosin Type V; Protein Isoforms/genetics; Protein Isoforms/physiology; Saccharomyces cerevisiae/cytology; Saccharomyces cerevisiae Proteins; Schizosaccharomyces pombe Proteins; Tropomyosin/genetics; Tropomyosin/physiology; Vesicular Transport Proteins; rab GTP-Binding Proteins |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
