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PMID:9822591
Citation |
Siniossoglou, S, Santos-Rosa, H, Rappsilber, J, Mann, M and Hurt, E (1998) A novel complex of membrane proteins required for formation of a spherical nucleus. EMBO J. 17:6449-64 |
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Abstract |
Two membrane proteins were identified through their genetic interaction with the nucleoporin Nup84p and shown to participate in nuclear envelope morphogenesis in yeast. One component is a known sporulation factor Spo7p, and the other, Nem1p, a novel protein whose C-terminal domain is conserved during eukaryotic evolution. Spo7p and Nem1p localize to the nuclear/ER membrane and behave biochemically as integral membrane proteins. Nem1p binds to Spo7p via its conserved C-terminal domain. Although cells without Spo7p or Nem1p are viable, they exhibit a drastically altered nuclear morphology with long, pore-containing double nuclear membrane extensions. These protrusions emanate from a core nucleus which contains the DNA, and penetrate deeply into the cytoplasm. Interestingly, not only Spo7(-) and Nem1(-), but also several nucleoporin mutants are defective in sporulation. Thus, Spo7p and Nem1p, which exhibit a strong genetic link to nucleoporins of the Nup84p complex, fulfil an essential role in formation of a spherical nucleus and meiotic division. |
Links |
PubMed PMC1170993 Online version:10.1093/emboj/17.22.6449 |
Keywords |
Amino Acid Sequence; Fungal Proteins/chemistry; Fungal Proteins/genetics; Fungal Proteins/metabolism; Membrane Proteins/chemistry; Membrane Proteins/genetics; Membrane Proteins/isolation & purification; Membrane Proteins/metabolism; Molecular Sequence Data; Nuclear Envelope/metabolism; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae/metabolism; Saccharomyces cerevisiae/physiology; Saccharomyces cerevisiae Proteins; Sequence Homology, Amino Acid; Spores, Fungal |
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