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PMID:9791031
| Citation |
Becker, S, Rinne, C, Hofsäss, U, Klenk, HD and Mühlberger, E (1998) Interactions of Marburg virus nucleocapsid proteins. Virology 249:406-17 |
|---|---|
| Abstract |
In this study, the components of Marburg virus nucleocapsid complex were determined, and interactions between the compounds were investigated. Using salt dissociation of isolated virions, four proteins (NP, VP35, VP30, and L) remained attached to the core complex. Same proteins were detected intracellularly to be localized in MBGV-induced inclusion bodies, which are presumed to represent areas of nucleocapsid formation. To investigate interactions between the four proteins, immunofluorescence analysis of coexpressed proteins was carried out. Complexes between NP-VP35 and NP-VP30 were formed, which was demonstrated by redistribution of VP35 and VP30 into NP-induced inclusion bodies. Furthermore, complexes between L and VP35 were detected by coimmunoprecipitation. Using deletion mutants of L, the binding site of VP35 on L could be restricted to the N-terminal 530 amino-acid residues. Coexpression of NP, VP35, and L led to the formation of a triple complex where VP35 linked NP and L. The detected complexes are presumed to represent the key components of the MBGV transcription and replication machinery. |
| Links |
PubMed Online version:10.1006/viro.1998.9328 |
| Keywords |
Animals; Base Sequence; Cercopithecus aethiops; HeLa Cells; Humans; Inclusion Bodies, Viral/metabolism; Macromolecular Substances; Marburgvirus/genetics; Marburgvirus/metabolism; Microscopy, Fluorescence; Nucleocapsid Proteins/chemistry; Nucleocapsid Proteins/genetics; Nucleocapsid Proteins/metabolism; Plasmids/genetics; Precipitin Tests; Recombinant Proteins/chemistry; Recombinant Proteins/genetics; Recombinant Proteins/metabolism; Vero Cells; Viral Proteins/chemistry; Viral Proteins/genetics; Viral Proteins/metabolism; Viral Regulatory and Accessory Proteins |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0019013: viral nucleocapsid |
ECO:0000314: |
C |
Figure 1 shows that NP was present in a pellet containing nucleocapsid particles after salt dissociation, sucrose centrifugation, and separation by SDS-PAGE. |
complete | ||||
| GO:0016234: inclusion body |
ECO:0000314: |
C |
Figure 2 A, C, and E show NP inclusion bodies observed by immunofluorescence with several different monoclonal antibodies. |
complete | ||||
|
part_of |
GO:0019013: viral nucleocapsid |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0019013: viral nucleocapsid |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019013: viral nucleocapsid |
ECO:0000314: |
C |
Figure 1 shows that VP 35 was present in a pellet containing nucleocapsid particles after salt dissociation, sucrose centrifugation, and separation by SDS-PAGE. |
complete | ||||
| GO:0016234: inclusion body |
ECO:0000314: |
C |
Figure 2 B shows VP 35 inclusion bodies observed by immunofluorescence with several different monoclonal antibodies. |
complete | ||||
|
part_of |
GO:0019013: viral nucleocapsid |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019013: viral nucleocapsid |
ECO:0000314: |
C |
Figure 1 shows that VP 30 was present in a pellet containing nucleocapsid particles after salt dissociation, sucrose centrifugation, and separation by SDS-PAGE. |
complete | ||||
| GO:0016234: inclusion body |
ECO:0000314: |
C |
Figure 2 D show VP 30 inclusion bodies observed by immunofluorescence with several different monoclonal antibodies. |
complete | ||||
|
part_of |
GO:0019013: viral nucleocapsid |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
| GO:0019013: viral nucleocapsid |
ECO:0000314: |
C |
Figure 1 shows that L was present in a pellet containing nucleocapsid particles after salt dissociation, sucrose centrifugation, and separation by SDS-PAGE. |
complete | ||||
See also
References
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