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PMID:9755172
| Citation |
Lefebvre, V, Li, P and de Crombrugghe, B (1998) A new long form of Sox5 (L-Sox5), Sox6 and Sox9 are coexpressed in chondrogenesis and cooperatively activate the type II collagen gene. EMBO J. 17:5718-33 |
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| Abstract |
Transcripts for a new form of Sox5, called L-Sox5, and Sox6 are coexpressed with Sox9 in all chondrogenic sites of mouse embryos. A coiled-coil domain located in the N-terminal part of L-Sox5, and absent in Sox5, showed >90% identity with a similar domain in Sox6 and mediated homodimerization and heterodimerization with Sox6. Dimerization of L-Sox5/Sox6 greatly increased efficiency of binding of the two Sox proteins to DNA containing adjacent HMG sites. L-Sox5, Sox6 and Sox9 cooperatively activated expression of the chondrocyte differentiation marker Col2a1 in 10T1/2 and MC615 cells. A 48 bp chondrocyte-specific enhancer in this gene, which contains several HMG-like sites that are necessary for enhancer activity, bound the three Sox proteins and was cooperatively activated by the three Sox proteins in non-chondrogenic cells. Our data suggest that L-Sox5/Sox6 and Sox9, which belong to two different classes of Sox transcription factors, cooperate with each other in expression of Col2a1 and possibly other genes of the chondrocytic program. |
| Links |
PubMed PMC1170900 Online version:10.1093/emboj/17.19.5718 |
| Keywords |
Amino Acid Sequence; Animals; Cartilage/embryology; Cell Differentiation; Chondrogenesis/genetics; Collagen/biosynthesis; Collagen/genetics; DNA, Complementary/genetics; DNA-Binding Proteins/biosynthesis; Dimerization; Enhancer Elements, Genetic; Gene Expression Regulation, Developmental; High Mobility Group Proteins/biosynthesis; Mice; Molecular Sequence Data; Nuclear Proteins/biosynthesis; Protein Binding; Protein Structure, Secondary; RNA, Messenger/genetics; SOX9 Transcription Factor; SOXD Transcription Factors; Sequence Homology, Amino Acid; Transcription Factors/biosynthesis |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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See also
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