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PMID:9735350
| Citation |
Shinwari, ZK, Nakashima, K, Miura, S, Kasuga, M, Seki, M, Yamaguchi-Shinozaki, K and Shinozaki, K (1998) An Arabidopsis gene family encoding DRE/CRT binding proteins involved in low-temperature-responsive gene expression. Biochem. Biophys. Res. Commun. 250:161-70 |
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| Abstract |
In higher plants, a cis-acting element, DRE/CRT, is involved in gene expression responsive to drought and low-temperature stress. To understand signal transduction pathways from the cold stress signal to gene expression, we characterized a gene family for DRE/CRT-binding proteins DREB1A and CBF1 in Arabidopsis thaliana. DREB1A and CBF1 were shown to be involved in low-temperature-responsive gene expression. We screened an Arabidopsis genomic DNA library with the cDNA fragment of DREB1A as a probe and isolated DREB1A and 2 related genes, DREB1B (= CBF1) and DREB1C. These were arrayed in the order B, A, C in an 8.7 kb region of Arabidopsis chromosome 4. Northern blot analysis using gene-specific probes showed that the 3 DREB1 genes are induced mainly by cold stress but not by osmotic stress in leaves, roots, and stems. Several conserved sequences were found in the promoter regions of all 3 genes. The beta-glucuronidase (GUS) reporter gene driven by the DREB1 promoters was induced at transcriptional level by low temperature in transgenic Arabidopsis plants. |
| Links |
PubMed Online version:10.1006/bbrc.1998.9267 |
| Keywords |
Amino Acid Sequence; Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis/physiology; Arabidopsis Proteins; Base Sequence; Cold Temperature; DNA-Binding Proteins/genetics; Gene Expression Regulation, Plant; Molecular Sequence Data; Multigene Family; Plants, Genetically Modified; RNA, Messenger/genetics; RNA, Messenger/metabolism; Sequence Homology, Nucleic Acid; Trans-Activators/genetics; Transcription Factors/genetics |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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