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PMID:9647753
| Citation |
Sakakibara, Y, Yanagisawa, K, Takami, Y, Nakayama, T, Suiko, M and Liu, MC (1998) Molecular cloning, expression, and functional characterization of novel mouse sulfotransferases. Biochem. Biophys. Res. Commun. 247:681-6 |
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| Abstract |
Nucleotide sequences of two mouse cDNAs encoding new sulfotransferase enzymes were determined. Analysis of the deduced amino acid sequences revealed that one represents a novel member of the phenol sulfotransferase family and the other is highly homologous to human SULT2B1 hydroxysteroid sulfotransferases. The recombinant enzymes, transiently expressed in COS-7 cells, were characterized with respect to their substrate specificity using a variety of substrates for different types of sulfotransferases. The tissue-specific expression of these two new mouse sulfotransferases was examined by Northern blot analysis. |
| Links |
PubMed Online version:10.1006/bbrc.1998.8872 |
| Keywords |
Amino Acid Sequence; Animals; Arylsulfotransferase/chemistry; COS Cells; Cloning, Molecular; Evolution, Molecular; Mice; Microsatellite Repeats/genetics; Molecular Sequence Data; RNA, Messenger/metabolism; Recombinant Proteins/chemistry; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Substrate Specificity; Sulfotransferases/chemistry; Transfection/genetics |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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See also
References
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