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PMID:9642267
| Citation |
Borggrefe, T, Wabl, M, Akhmedov, AT and Jessberger, R (1998) A B-cell-specific DNA recombination complex. J. Biol. Chem. 273:17025-35 |
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| Abstract |
We have purified and biochemically characterized a multiprotein complex designated SWAP. In a DNA transfer assay, SWAP preferentially recombines ("swaps") sequences derived from Ig heavy chain switch regions. We identified four of the proteins in the SWAP complex: B23 (nucleophosmin), C23 (nucleolin), poly(ADP-ribose) polymerase (PARP), and SWAP-70. The first three are proteins known to be present in most cells. B23 promotes single-strand DNA reannealing and the formation of joint molecules in a D-loop assay between homologous, but also between Smu and Sgamma sequences. SWAP-70 is a novel protein of 70 kDa. Its cDNA was cloned and sequenced, and the protein was overexpressed in Escherichia coli. SWAP-70 protein expression was found only in B lymphocytes that had been induced to switch to various Ig isotypes and in switching B-cell lines. SWAP-70 is a nuclear protein, has a weak affinity for DNA, binds ATP, and forms specific, high affinity complexes with B23, C23, and poly(ADP-ribose) polymerase. These findings are consistent with SWAP being the long elusive "switch recombinase" and with SWAP-70 being the specific recruiting element that assembles the switch recombinase from universal components. |
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| Keywords |
Adenosine Triphosphate/metabolism; Amino Acid Sequence; Animals; B-Lymphocytes/metabolism; Base Sequence; Cloning, Molecular; DNA, Recombinant/genetics; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Escherichia coli/genetics; Guanine Nucleotide Exchange Factors; Hot Temperature; Kinetics; Mice; Mice, Inbred C57BL; Molecular Sequence Data; Nuclear Proteins/genetics; Nuclear Proteins/metabolism; Phosphoproteins/metabolism; Poly(ADP-ribose) Polymerases/metabolism; Protein Binding; Recombination, Genetic |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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