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PMID:9630982
| Citation |
Sparks, AB, Hoffman, NG, McConnell, SJ, Fowlkes, DM and Kay, BK (1996) Cloning of ligand targets: systematic isolation of SH3 domain-containing proteins. Nat. Biotechnol. 14:741-4 |
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| Abstract |
Based on the prevalence of modular protein domains, such as Src homology domain 3 and 2 (SH3 and SH2), among important signaling molecules, we have sought to identify new SH3 domain-containing proteins. However, modest sequence similarity among these domains restricts the use of DNA-based methods for this purpose. To circumvent this limitation, we have developed a functional screen that permits the rapid cloning of modular domains based on their ligand-binding activity. Using operationally defined SH3 ligands from combinatorial peptide libraries, we screened a series of mouse and human cDNA expression libraries. We found that 69 of the 74 clones isolated encode at least one SH3 domain. These clones encode 18 different SH3-containing proteins, 10 of which have not been described previously. The isolation of entire repertoires of modular domain-containing proteins will prove invaluable in genome analysis and in bringing new targets into drug discovery programs. |
| Links |
PubMed Online version:10.1038/nbt0696-741 |
| Keywords |
Amino Acid Sequence; Animals; Cloning, Molecular; Humans; Ligands; Mice; Molecular Sequence Data; Protein Binding; Proteins/chemistry; Proteins/genetics; Proteins/metabolism; Sequence Homology, Amino Acid; src Homology Domains |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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