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Yasuda, T, Morimatsu, K, Horii, T, Nagata, T and Ohmori, H (1998) Inhibition of Escherichia coli RecA coprotease activities by DinI. EMBO J. 17:3207-16
In Escherichia coli, the SOS response is induced upon DNA damage and results in the enhanced expression of a set of genes involved in DNA repair and other functions. The initial step, self-cleavage of the LexA repressor, is promoted by the RecA protein which is activated upon binding to single-stranded DNA. In this work, induction of the SOS response by the addition of mitomycin C was found to be prevented by overexpression of the dinI gene. dinI is an SOS gene which maps at 24.6 min of the E.coli chromosome and encodes a small protein of 81 amino acids. Immunoblotting analysis with anti-LexA antibodies revealed that LexA did not undergo cleavage in dinI-overexpressed cells after UV irradiation. In addition, the RecA-dependent conversion of UmuD to UmuD' (the active form for mutagenesis) was also inhibited in dinI-overexpressed cells. Conversely, a dinI-deficient mutant showed a slightly faster and more extensive processing of UmuD and hence higher mutability than the wild-type. Finally, we demonstrated, by using an in vitro reaction with purified proteins, that DinI directly inhibits the ability of RecA to mediate self-cleavage of UmuD.
Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; DNA-Directed DNA Polymerase; Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Genes, Bacterial; Mutagenesis; Rec A Recombinases/antagonists & inhibitors; Rec A Recombinases/genetics; Rec A Recombinases/metabolism; Recombination, Genetic; SOS Response (Genetics)/genetics
|Gene product||Qualifier||GO Term||Evidence Code||with/from||Aspect||Extension||Notes||Status|
|GO:0009432: SOS response||
DinI is induced during the SOS response, see figures 5 and 6 as well as table 2.
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