PMID:9536054

Citation	Kaye, C, Neven, L, Hofig, A, Li, QB, Haskell, D and Guy, C (1998) Characterization of a gene for spinach CAP160 and expression of two spinach cold-acclimation proteins in tobacco. Plant Physiol. 116:1367-77
Abstract	The cDNA sequence for CAP160, an acidic protein previously linked with cold acclimation in spinach (Spinacia oleracea L.), was characterized and found to encode a novel acidic protein of 780 amino acids having very limited homology to a pair of Arabidopsis thaliana stress-regulated proteins, rd29A and rd29B. The lack of similarity in the structural organization of the spinach and Arabidopsis genes highlights the absence of a high degree of conservation of this cold-stress gene across taxonomic boundaries. The protein has several unique motifs that may relate to its function during cold stress. Expression of the CAP160 mRNA was increased by low-temperature exposure and water stress in a manner consistent with a probable function during stresses that involve dehydration. The coding sequences for CAP160 and CAP85, another spinach cold-stress protein, were introduced into tobacco (Nicotiana tabacum) under the control of the 35S promoter using Agrobacterium tumefaciens-based transformation. Tobacco plants expressing the proteins individually or coexpressing both proteins were evaluated for relative freezing-stress tolerance. The killing temperature for 50% of the cells of the transgenic plants was not different from that of the wild-type plants. As determined by a more sensitive time/temperature kinetic study, plants expressing the spinach proteins had slightly lower levels of electrolyte leakage than wild-type plants, indicative of a small reduction of freezing-stress injury. Clearly, the heterologous expression of two cold-stress proteins had no profound influence on stress tolerance, a result that is consistent with the quantitative nature of cold-stress-tolerance traits.
Links	PubMed PMC35044
Keywords	Adaptation, Physiological/genetics; Amino Acid Sequence; Base Sequence; Cold Temperature; DNA, Complementary; Freezing; Molecular Sequence Data; Plant Proteins/genetics; Plants, Genetically Modified; Plants, Toxic; RNA, Messenger/genetics; RNA, Messenger/metabolism; Sequence Homology, Amino Acid; Spinacia oleracea/genetics; Spinacia oleracea/physiology; Tobacco/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
SPIOL:O50054	involved_in	GO:0009631: cold acclimation	ECO:0000314: direct assay evidence used in manual assertion		P		Seeded From UniProt	complete
SPIOL:O50054		GO:0009631: cold acclimation	ECO:0000314:		P		Fig 12 shows expression of spinach CAP160 in tobacco affects cold acclimation	complete CACAO 7294

References

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