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PMID:9514871
| Citation |
Ishido, S, Fujita, T and Hotta, H (1998) Complex formation of NS5B with NS3 and NS4A proteins of hepatitis C virus. Biochem. Biophys. Res. Commun. 244:35-40 |
|---|---|
| Abstract |
At present, the mechanism of replication of the HCV genome remains unclear. Recently, NS5B and NS3 of HCV have been shown to exhibit RNA-dependent RNA polymerase and helicase activities, respectively, both of which are indispensable for virus RNA replication. In this study, we examined the complex formation of NS5B with NS3 and NS4A, a cofactor for NS3. We show here that NS5B forms a complex with NS3 through an amino-terminal portion of NS3. The NS3-NS5B complex formation took place both in the presence and absence of NS4A. We also demonstrate that NS5B form a complex with NS4A in the absence of NS3. These results suggest that NS3, NS4A and NS5B interact with each other to form a complex that functions as part of the replication machinery of HCV. |
| Links |
PubMed Online version:10.1006/bbrc.1998.8202 |
| Keywords |
Amino Acid Sequence; Animals; COS Cells; Cell Nucleus/enzymology; Cell Nucleus/virology; Cytoplasm/enzymology; Cytoplasm/virology; HeLa Cells; Hepacivirus/enzymology; Humans; Multienzyme Complexes/metabolism; RNA Nucleotidyltransferases/metabolism; RNA Replicase/metabolism; Subcellular Fractions/enzymology; Subcellular Fractions/virology; Viral Nonstructural Proteins/metabolism |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
|
part_of |
GO:0042025: host cell nucleus |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0030430: host cell cytoplasm |
ECO:0000314: direct assay evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0042025: host cell nucleus |
ECO:0000315: mutant phenotype evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
|
part_of |
GO:0030430: host cell cytoplasm |
ECO:0000315: mutant phenotype evidence used in manual assertion |
C |
Seeded From UniProt |
complete | |||
Notes
See also
References
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