GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:9503607
| Citation |
Frazzon, J and Schrank, IS (1998) Sequencing and complementation analysis of the nifUSV genes from Azospirillum brasilense. FEMS Microbiol. Lett. 159:151-8 |
|---|---|
| Abstract |
The functionality of nitrogenase in diazotrophic bacteria is dependent upon nif genes other than the structural nifH, D, and K genes which encode the enzyme subunit proteins. Such genes are involved in the activation of nif gene expression, maturation of subunit proteins, cofactor biosynthesis, and electron transport. In this work, approximately 5500 base pairs located within the major nif gene cluster of Azospirillum brasilense Sp7 have been sequenced. The deduced open reading frames were compared to the nif gene products of Azotobacter vinelandii and other diazotrophs. This analysis indicates the presence of five ORFs encoding ORF2, nifU, nifS, nifV, and ORF4 in the same sequential organization as found in other organisms. Consensus sigma 54 and NifA binding sites are present in the putative promoter region upstream of ORF2 in the A. brasilense sequence. The nifV gene of A. brasilense but not nifU or nifS complemented corresponding mutants strains of A. vinelandii. |
| Links | |
| Keywords |
Amino Acid Sequence; Azospirillum/genetics; Genes, Bacterial; Genetic Complementation Test; Molecular Sequence Data; Nitrogen Fixation/genetics; Open Reading Frames |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
See also
References
See Help:References for how to manage references in GONUTS.
