PMID:9497323

Citation	Vatcher, GP, Thacker, CM, Kaletta, T, Schnabel, H, Schnabel, R and Baillie, DL (1998) Serine hydroxymethyltransferase is maternally essential in Caenorhabditis elegans. J. Biol. Chem. 273:6066-73
Abstract	The mel-32 gene in the free living soil nematode Caenorhabditis elegans encodes a serine hydroxymethyltransferase (SHMT) isoform. Seventeen ethylmethanesulfonate (EMS)-induced mutant alleles of mel-32(SHMT) have been generated, each of which causes a recessive maternal effect lethal phenotype. Animals homozygous for the SHMT mutations have no observable mutant phenotype, but their offspring display an embryonic lethal phenotype. The Mel-32 phenotype has been rescued with a transgenic array containing only mel-32(SHMT) genomic DNA. Heteroduplex analysis of the 17 alleles allowed 14 of the mutations to be positioned to small regions. Subsequent sequence analysis has shown that 16 of the alleles alter highly conserved amino acids, while one allele introduces a stop codon that truncates two thirds of the predicted protein. mel-32(SHMT) has a 55-60% identity at the amino acid level with both isoforms of SHMT found in yeast and humans and a 50% identity with the Escherichia coli isoform. The C. elegans mel-32 mutation represents the first case where SHMT has been shown to be an essential gene.
Links	PubMed
Keywords	Amino Acid Sequence; Animals; Base Sequence; Caenorhabditis elegans/enzymology; Caenorhabditis elegans/genetics; Cloning, Molecular; Female; Genes, Helminth; Genes, Lethal; Glycine Hydroxymethyltransferase/genetics; Helminth Proteins/genetics; Heterozygote; Homozygote; Isoenzymes/genetics; Male; Molecular Sequence Data; Mutagenesis; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Sex Characteristics
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.