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PMID:9492266

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Citation

Vörös, K, Feussner, I, Kühn, H, Lee, J, Graner, A, Löbler, M, Parthier, B and Wasternack, C (1998) Characterization of a methyljasmonate-inducible lipoxygenase from barley (Hordeum vulgare cv. Salome) leaves. Eur. J. Biochem. 251:36-44

Abstract

We found three methyl jasmonate-induced lipoxygenases with molecular masses of 92 kDa, 98 kDa, and 100 kDa (LOX-92, -98 and -100) [Feussner, I., Hause, B., Vörös, K., Parthier, B. & Wasternack, C. (1995) Plant J. 7, 949-957]. At least two of them (LOX-92 and LOX-100), were shown to be localized within chloroplasts of barley leaves. Here, we describe the isolation of a cDNA (3073 bp) coding for LOX-100, a protein of 936 amino acid residues and a molecular mass of 106 kDa. By sequence comparison this lipoxygenase could be identified as LOX2-type lipoxygenase and was therefore designated LOX2:Hv:1. The recombinant lipoxygenase was expressed in Escherichia coli and characterized as linoleate 13-LOX and arachidonate 15-LOX, respectively. The enzyme exhibited a pH optimum around pH 7.0 and a moderate substrate preference for linoleic acid. The gene was transiently expressed after exogenous application of jasmonic acid methyl ester with a maximum between 12 h and 18 h. Its expression was not affected by exogenous application of abscisic acid. Also a rise of endogenous jasmonic acid resulting from sorbitol stress did not induce LOX2:Hv:1, suggesting a separate signalling pathway compared with other jasmonate-induced proteins of barley. The properties of LOX2:Hv:1 are discussed in relation to its possible involvement in jasmonic acid biosynthesis and other LOX forms of barley identified so far.

Links

PubMed

Keywords

Acetates/pharmacology; Amino Acid Sequence; Base Sequence; Chromosome Mapping; Cyclopentanes/pharmacology; DNA, Complementary/isolation & purification; Gene Expression Regulation, Plant/drug effects; Hordeum/enzymology; Lipoxygenase/drug effects; Lipoxygenase/genetics; Lipoxygenase/metabolism; Molecular Sequence Data; Oxylipins; Plant Growth Regulators/pharmacology; Plant Leaves/enzymology; Sequence Analysis, DNA; Sorbitol/pharmacology

Significance

Annotations

Gene product Qualifier GO Term Evidence Code with/from Aspect Extension Notes Status

HORVU:LOX21

enables

GO:0016165: linoleate 13S-lipoxygenase activity

ECO:0000314: direct assay evidence used in manual assertion

F

Seeded From UniProt

complete

HORVU:LOX21

involved_in

GO:0009753: response to jasmonic acid

ECO:0000314: direct assay evidence used in manual assertion

P

Seeded From UniProt

complete

HORVU:LOX21

GO:0016165: lipoxygenase activity

ECO:0000314:

F

LOX2:Hv:1 protein was expressed in Escherichia coli. Lipoxygenase activity in the bacterial extracts was determined spectrophotometrically using linoleic, linolenic, and arachidonic acid as substrates. As seen in figure 5, HPLC was used to demonstrate products of the oxygenation of arachidonic acid.

complete
CACAO 8403

HORVU:LOX21

GO:0009753: response to jasmonic acid stimulus

ECO:0000314:

P

Figure 7B

complete
CACAO 8404


See also

References

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