GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:9492266
Citation |
Vörös, K, Feussner, I, Kühn, H, Lee, J, Graner, A, Löbler, M, Parthier, B and Wasternack, C (1998) Characterization of a methyljasmonate-inducible lipoxygenase from barley (Hordeum vulgare cv. Salome) leaves. Eur. J. Biochem. 251:36-44 |
---|---|
Abstract |
We found three methyl jasmonate-induced lipoxygenases with molecular masses of 92 kDa, 98 kDa, and 100 kDa (LOX-92, -98 and -100) [Feussner, I., Hause, B., Vörös, K., Parthier, B. & Wasternack, C. (1995) Plant J. 7, 949-957]. At least two of them (LOX-92 and LOX-100), were shown to be localized within chloroplasts of barley leaves. Here, we describe the isolation of a cDNA (3073 bp) coding for LOX-100, a protein of 936 amino acid residues and a molecular mass of 106 kDa. By sequence comparison this lipoxygenase could be identified as LOX2-type lipoxygenase and was therefore designated LOX2:Hv:1. The recombinant lipoxygenase was expressed in Escherichia coli and characterized as linoleate 13-LOX and arachidonate 15-LOX, respectively. The enzyme exhibited a pH optimum around pH 7.0 and a moderate substrate preference for linoleic acid. The gene was transiently expressed after exogenous application of jasmonic acid methyl ester with a maximum between 12 h and 18 h. Its expression was not affected by exogenous application of abscisic acid. Also a rise of endogenous jasmonic acid resulting from sorbitol stress did not induce LOX2:Hv:1, suggesting a separate signalling pathway compared with other jasmonate-induced proteins of barley. The properties of LOX2:Hv:1 are discussed in relation to its possible involvement in jasmonic acid biosynthesis and other LOX forms of barley identified so far. |
Links | |
Keywords |
Acetates/pharmacology; Amino Acid Sequence; Base Sequence; Chromosome Mapping; Cyclopentanes/pharmacology; DNA, Complementary/isolation & purification; Gene Expression Regulation, Plant/drug effects; Hordeum/enzymology; Lipoxygenase/drug effects; Lipoxygenase/genetics; Lipoxygenase/metabolism; Molecular Sequence Data; Oxylipins; Plant Growth Regulators/pharmacology; Plant Leaves/enzymology; Sequence Analysis, DNA; Sorbitol/pharmacology |
edit table |
Significance
Annotations
Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
---|---|---|---|---|---|---|---|---|
enables |
GO:0016165: linoleate 13S-lipoxygenase activity |
ECO:0000314: direct assay evidence used in manual assertion |
F |
Seeded From UniProt |
complete | |||
involved_in |
GO:0009753: response to jasmonic acid |
ECO:0000314: direct assay evidence used in manual assertion |
P |
Seeded From UniProt |
complete | |||
GO:0016165: lipoxygenase activity |
ECO:0000314: |
F |
LOX2:Hv:1 protein was expressed in Escherichia coli. Lipoxygenase activity in the bacterial extracts was determined spectrophotometrically using linoleic, linolenic, and arachidonic acid as substrates. As seen in figure 5, HPLC was used to demonstrate products of the oxygenation of arachidonic acid. |
complete | ||||
GO:0009753: response to jasmonic acid stimulus |
ECO:0000314: |
P |
Figure 7B |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.