PMID:9435261

Citation	Häse, CC and Mekalanos, JJ (1998) TcpP protein is a positive regulator of virulence gene expression in Vibrio cholerae. Proc. Natl. Acad. Sci. U.S.A. 95:730-4
Abstract	The production of several virulence factors in Vibrio cholerae O1, including cholera toxin and the pilus colonization factor TCP (toxin-coregulated pilus), is strongly influenced by environmental conditions. To specifically identify membrane proteins involved in these signal transduction events, we examined a transposon library of V. cholerae generated by Tnbla mutagenesis for cells that produce TCP when grown under various nonpermissive conditions. To select for TCP-producing cells we used the recently described bacteriophage CTX phi-Kan, which uses TCP as its receptor and carries a gene encoding resistance to kanamycin. Among the isolated mutants was a transposon insertion in a gene homologous to nqrB from Vibrio alginolyticus, which encodes a subunit of a Na(+)-translocating NADH:ubiquinone oxidoreductase, and tcpI, encoding a chemo-receptor previously implicated in the negative regulation of TCP production. A third transposon mutant had an insertion in tcpP, which is in an operon with tcpH, a known positive regulator of TCP production. However, TcpP was shown to be essential for TCP production in V. cholerae, as a tcpP-deletion strain was deficient in pili production. The amino-terminal region of TcpP shows sequence homology to the DNA-binding domains of several regulatory proteins, including ToxR from V. cholerae and PsaE from Yersinia pestis. Like ToxR, TcpP activates transcription of the toxT gene, an essential activator of tcp operon transcription. Furthermore, TcpH, with its large periplasmic domain and inner membrane anchor, has a structure similar to that of ToxS and was shown to enhance the activity of TcpP. We propose that TcpP/TcpH constitute a pair of regulatory proteins functionally similar to ToxR/ToxS and PsaE/PsaF that are required for toxT transcription in V. cholerae.
Links	PubMed PMC18489
Keywords	Amino Acid Sequence; Bacterial Proteins/genetics; Gene Expression Regulation, Bacterial; Molecular Sequence Data; Sequence Alignment; Transcription Factors; Vibrio cholerae/genetics; Vibrio cholerae/pathogenicity; Virulence/genetics
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
VIBCH:TCPP	involved_in	GO:0045893: positive regulation of transcription, DNA-templated	ECO:0000316: genetic interaction evidence used in manual assertion	UniProtKB:A5F398	P		Seeded From UniProt	complete
VIBCH:TCPP		GO:0045893: positive regulation of transcription, DNA-dependent	ECO:0000316:	UniProtKB:A5F398	P		Figure 3.	complete CACAO 2201

References

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