GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:9425043
| Citation |
Martín-Nieto, J and Villalobo, A (1998) The human epidermal growth factor receptor contains a juxtamembrane calmodulin-binding site. Biochemistry 37:227-36 |
|---|---|
| Abstract |
A ligand-insensitive form of the human epidermal growth factor receptor (EGFR) was enriched by Ca2+-dependent calmodulin-affinity chromatography purification. The basic amphiphilic segment Arg645-Arg-Arg-His-Ile-Val-Arg-Lys-Arg-Thr654-Leu-Arg-Arg-Le u-Leu-Gln 660, located within the cytoplasmic juxtamembrane domain of this receptor, was purified as a fusion protein with glutathione S-transferase and shown to bind calmodulin in a Ca2+-dependent manner. An apparent dissociation constant of 0.4 microM calmodulin (Kd'(CaM)) and an apparent affinity constant of 0.5 microM free Ca2+ (Ka'(Ca)) were measured for this binding process. Binding of calmodulin at the juxtamembrane site prevented the phosphorylation of residue Thr-654 by protein kinase C, and an apparent inhibition constant of 0.5-1 microM calmodulin (Ki'(CaM)) was determined. Conversely, phosphorylation of this site by protein kinase C prevented its subsequent interaction with calmodulin. We therefore propose that cross talk between signaling pathways mediated by calmodulin and protein kinase C occurs at the juxtamembrane domain of the EGFR. This calmodulin-binding sequence is highly conserved among protein tyrosine kinases of the vertebrate EGFR family. |
| Links |
PubMed Online version:10.1021/bi971765v |
| Keywords |
3T3 Cells; Amino Acid Sequence; Animals; Binding Sites; Calmodulin/metabolism; Calmodulin-Binding Proteins/metabolism; Cell Membrane/enzymology; Cell Membrane/metabolism; Chromatography, Affinity; Cytoplasm/metabolism; Humans; Mice; Molecular Sequence Data; Phosphorylation; Protein Kinase C/metabolism; Receptor, Epidermal Growth Factor/chemistry; Receptor, Epidermal Growth Factor/isolation & purification; Receptor, Epidermal Growth Factor/metabolism; Threonine/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0005515: protein binding |
IPI: Inferred from Physical Interaction: UniProtKB:Q29376 |
F |
| |||||
| GO:0005515: protein binding |
IPI: Inferred from Physical Interaction: UniProtKB:P62157 |
F |
| |||||
See also
References
See Help:References for how to manage references in GONUTS.
