GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:9422589
| Citation |
Allen, AG, Isobe, T and Maskell, DJ (1998) Identification and cloning of waaF (rfaF) from Bordetella pertussis and use to generate mutants of Bordetella spp. with deep rough lipopolysaccharide. J. Bacteriol. 180:35-40 |
|---|---|
| Abstract |
A DNA locus from Bordetella pertussis capable of reconstituting lipopolysaccharide (LPS) O-antigen biosynthesis in Salmonella typhimurium SL3789 (rfaF511) has been isolated, by using selection with the antibiotic novobiocin. DNA within the locus encodes a protein with amino acid sequence similarity to heptosyltransferase II, encoded by waaF (previously rfaF) in other gram-negative bacteria. Mutation of this gene in B. pertussis, Bordetella parapertussis, and Bordetella bronchiseptica by allelic exchange generated bacteria with deep rough LPS phenotypes consistent with the proposed function of the gene as an inner core heptosyltransferase. These are the first LPS mutants generated in B. parapertussis and B. bronchiseptica and the first deep rough mutants of any of the bordetellae. |
| Links | |
| Keywords |
Amino Acid Sequence; Bordetella/enzymology; Bordetella/genetics; Bordetella pertussis/enzymology; Bordetella pertussis/genetics; Cloning, Molecular; Genes, Bacterial/genetics; Genetic Complementation Test; Glycosyltransferases/genetics; Lipopolysaccharides/biosynthesis; Molecular Sequence Data; Mutation/physiology; O Antigens/biosynthesis; Phenotype; Sequence Analysis, DNA; Species Specificity |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO Term | Evidence Code | with/from | Aspect | Extension | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| GO:0008920: lipopolysaccharide heptosyltransferase activity |
ECO:0000315: |
F |
Figure 4 shows that the mutant waaF gene of B. pertussis produces a lipopolysaccharide that has a deep, rough phenotype, which leads to the explanation that waaF finds its function in lipopolysaccharide biosynthesis. |
complete | ||||
See also
References
See Help:References for how to manage references in GONUTS.
