PMID:9353924

Citation	Müller, J, Schiel, S, Ordal, GW and Saxild, HH (1997) Functional and genetic characterization of mcpC, which encodes a third methyl-accepting chemotaxis protein in Bacillus subtilis. Microbiology (Reading, Engl.) 143 ( Pt 10):3231-40
Abstract	A 3135 bp DNA segment downstream of the spl gene on the Bacillus subtilis chromosome was cloned and its nucleotide sequence determined. An open reading frame capable of encoding a putative protein of 654 amino acids with a calculated molecular mass of 72.1 kDa was identified. The deduced amino acid sequence was similar to the McpA and McpB proteins of B. subtilis. McpA and McpB encode different methyl-accepting chemotaxis proteins (MCPs). A mutant strain containing an antibiotic resistance DNA cassette inserted into the region containing the MCP-like reading frame suffered a complete loss of taxis to the amino acids cysteine, proline, threonine, glycine, serine, lysine, valine and arginine. The open reading frame was designated mcpC. The wild-type and an mcpC mutant strain were analysed for their content of methylated proteins and it was found that mcpC encodes a methylated membrane protein that has previously been designated H3. These results show that mcpC encodes a third MCP in B. subtilis. The transcription start site upstream of the mcpC gene was determined by primer extension analysis and it was found to be preceded by a potential promoter sequence that is recognized by the sigma D form of RNA polymerase. The level of beta-galactosidase expressed from a transcriptional mcpC-lacZ fusion was increased threefold when cells entered the stationary phase. No beta-galactosidase could be detected in a sigD genetic background.
Links	PubMed
Keywords	Amino Acid Sequence; Bacillus subtilis/genetics; Bacillus subtilis/physiology; Bacterial Proteins/genetics; Base Sequence; Chemotaxis/genetics; Chromosome Mapping; Cloning, Molecular; DNA, Bacterial/genetics; Gene Expression; Genes, Bacterial; Membrane Proteins/genetics; Molecular Sequence Data; Mutation; Open Reading Frames
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Annotations

Gene product	Qualifier	GO Term	Evidence Code	with/from	Aspect	Extension	Notes	Status
BACSU:MCPC		GO:0050918: positive chemotaxis	ECO:0000315:		P		Table 2	complete CACAO 10395
BACSU:MCPC	involved_in	GO:0050918: positive chemotaxis	ECO:0000315: mutant phenotype evidence used in manual assertion		P		Seeded From UniProt	complete

References

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