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PMID:9214640
Citation |
Gotta, M, Strahl-Bolsinger, S, Renauld, H, Laroche, T, Kennedy, BK, Grunstein, M and Gasser, SM (1997) Localization of Sir2p: the nucleolus as a compartment for silent information regulators. EMBO J. 16:3243-55 |
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Abstract |
In wild-type budding yeast strains, the proteins encoded by SIR3, SIR4 and RAP1 co-localize with telomeric DNA in a limited number of foci in interphase nuclei. Immunostaining of Sir2p shows that in addition to a punctate staining that coincides with Rap1 foci, Sir2p localizes to a subdomain of the nucleolus. The presence of Sir2p at both the spacer of the rDNA repeat and at telomeres is confirmed by formaldehyde cross-linking and immunoprecipitation with anti-Sir2p antibodies. In strains lacking Sir4p, Sir3p becomes concentrated in the nucleolus, by a pathway requiring SIR2 and UTH4, a gene that regulates life span in yeast. The unexpected nucleolar localization of Sir2p and Sir3p correlates with observed effects of sir mutations on rDNA stability and yeast longevity, defining a new site of action for silent information regulatory factors. |
Links |
PubMed PMC1169941 Online version:10.1093/emboj/16.11.3243 |
Keywords |
Antibodies, Fungal; Antibody Specificity; Cell Compartmentation; Cell Nucleolus/genetics; Cell Nucleolus/ultrastructure; DNA, Ribosomal; DNA-Binding Proteins/genetics; DNA-Binding Proteins/immunology; DNA-Binding Proteins/isolation & purification; Fluorescent Antibody Technique; Fungal Proteins/genetics; Fungal Proteins/immunology; Fungal Proteins/isolation & purification; GTP-Binding Proteins/immunology; GTP-Binding Proteins/isolation & purification; Histone Deacetylases; Models, Biological; Polymerase Chain Reaction; Precipitin Tests; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/ultrastructure; Silent Information Regulator Proteins, Saccharomyces cerevisiae; Sirtuin 2; Sirtuins; Telomere; Trans-Activators/genetics; Trans-Activators/immunology; Trans-Activators/isolation & purification; rap GTP-Binding Proteins |
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Significance
Annotations
Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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