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PMID:9177270
| Citation |
Sammar, M, Gulbins, E, Hilbert, K, Lang, F and Altevogt, P (1997) Mouse CD24 as a signaling molecule for integrin-mediated cell binding: functional and physical association with src-kinases. Biochem. Biophys. Res. Commun. 234:330-4 |
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| Abstract |
CD24 is a differentiation antigen expressed by murine hematopoietic and neural cells which is linked to the membrane via a glycosylphosphatidylinositol (GPI) anchor. In monocytic ESb-MP cells the molecule serves as a ligand for P-selectin and triggering with CD24 specific antibodies can activate VLA-5/L1-mediated cell adhesion in these cells. We report here that the aggregation is specific for CD24 and not seen with antibodies to the GPI-anchored molecule Thy-1. The Tyr-kinase inhibitor herbimycin can block the aggregation. We studied CD24 associated molecules that might be involved in signal transduction. Antibodymediated crosslinking of CD24 induced a rapid Tyr-phosphorylation of several cellular proteins in ESb-MP cells which correlated with an elevated activity of p56lck but not p60fyn or MAP-1 kinase. Several phosphorylated proteins were co-immunoprecipitated with CD24. Re-immunoprecipitation allowed the detection of p56lck, p56hck, and p54lyn but not p60fyn, PI-3k, or PLCgamma as a compenent of the CD24 detergent resistant complex. It is suggested that the CD24-associated kinases are involved in the activation of cell aggregation. |
| Links |
PubMed Online version:10.1006/bbrc.1997.6639 |
| Keywords |
Animals; Antibodies; Antigens, CD/chemistry; Antigens, CD/metabolism; Antigens, CD24; Cell Aggregation; Cell Line; Cross-Linking Reagents; Enzyme Activation; Integrins/metabolism; Lymphocyte Specific Protein Tyrosine Kinase p56(lck); Membrane Glycoproteins; Mice; Molecular Structure; Molecular Weight; Monocytes/cytology; Monocytes/immunology; Monocytes/metabolism; Phosphoproteins/chemistry; Phosphoproteins/metabolism; Phosphorylation; Signal Transduction; Tyrosine/chemistry; Tyrosine/metabolism; src-Family Kinases/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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