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PMID:9169132
| Citation |
Hendriksen, PJ, Hoogerbrugge, JW, Baarends, WM, de Boer, P, Vreeburg, JT, Vos, EA, van der Lende, T and Grootegoed, JA (1997) Testis-specific expression of a functional retroposon encoding glucose-6-phosphate dehydrogenase in the mouse. Genomics 41:350-9 |
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| Abstract |
The X-chromosomal gene glucose-6-phosphate dehydrogenase (G6pd) is known to be expressed in most cell types of mammalian species. In the mouse, we have detected a novel gene, designated G6pd-2, encoding a G6PD isoenzyme. G6pd-2 does not contain introns and appears to represent a retroposed gene. This gene is uniquely transcribed in postmeiotic spermatogenic cells in which the X-encoded G6pd gene is not transcribed. Expression of the G6pd-2 sequence in a bacterial system showed that the encoded product is an active enzyme. Zymogramic analysis demonstrated that recombinant G6PD-2, but not recombinant G6PD-1 (the X-chromosome-encoded G6PD), formed tetramers under reducing conditions. Under the same conditions, G6PD tetramers were also found in extracts of spermatids and spermatozoa, indicating the presence of G6pd-2-encoded isoenzyme in these cell types. G6pd-2 is one of the very few known expressed retroposons encoding a functional protein, and the presence of this gene is probably related to X chromosome inactivation during spermatogenesis. |
| Links |
PubMed Online version:10.1006/geno.1997.4673 |
| Keywords |
Amino Acid Sequence; Animals; Base Sequence; DNA/genetics; DNA Primers/genetics; Dosage Compensation, Genetic; Gene Expression; Glucosephosphate Dehydrogenase/genetics; Isoenzymes/genetics; Male; Mice; Molecular Sequence Data; Phylogeny; RNA, Messenger/genetics; RNA, Messenger/metabolism; Rats; Retroelements; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid; Spermatogenesis/genetics; Testis/enzymology; Tissue Distribution; X Chromosome/genetics |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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