GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:9150136
| Citation |
Kadosh, D and Struhl, K (1997) Repression by Ume6 involves recruitment of a complex containing Sin3 corepressor and Rpd3 histone deacetylase to target promoters. Cell 89:365-71 |
|---|---|
| Abstract |
Sin3 and Rpd3 negatively regulate a diverse set of yeast genes. A mouse Sin3-related protein is a transcriptional corepressor, and a human Rpd3 homolog is a histone deacetylase. Here, we show that Sin3 and Rpd3 are specifically required for transcriptional repression by Ume6, a DNA-binding protein that regulates genes involved in meiosis. A short region of Ume6 is sufficient to repress transcription, and this repression domain mediates a two-hybrid and physical interaction with Sin3. Coimmunoprecipitation and two-hybrid experiments indicate that Sin3 and Rpd3 are associated in a complex distinct from TFIID and Pol II holoenzyme. Rpd3 is specifically required for repression by Sin3, and artificial recruitment of Rpd3 results in repression. These results suggest that repression by Ume6 involves recruitment of a Sin3-Rpd3 complex and targeted histone deacetylation. |
| Links | |
| Keywords |
DNA-Binding Proteins/chemistry; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Fungal Proteins/chemistry; Fungal Proteins/genetics; Fungal Proteins/metabolism; Gene Expression Regulation, Enzymologic/physiology; Gene Expression Regulation, Fungal/physiology; Histone Deacetylases/genetics; Histone Deacetylases/metabolism; Multienzyme Complexes/physiology; Promoter Regions, Genetic/physiology; Protein Binding/physiology; Protein Structure, Tertiary; Repressor Proteins/genetics; Repressor Proteins/metabolism; Saccharomyces cerevisiae Proteins; Transcription Factors/chemistry; Transcription Factors/genetics; Transcription Factors/metabolism; Transcription, Genetic/physiology; Yeasts/enzymology; beta-Galactosidase/genetics; beta-Galactosidase/metabolism |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
