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PMID:9147327
| Citation |
Ito, I, Futai, K, Katagiri, H, Watanabe, M, Sakimura, K, Mishina, M and Sugiyama, H (1997) Synapse-selective impairment of NMDA receptor functions in mice lacking NMDA receptor epsilon 1 or epsilon 2 subunit. J. Physiol. (Lond.) 500 ( Pt 2):401-8 |
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| Abstract |
1. We have explored the effects of targeted disruption of the N-methyl-D-aspartate (NMDA) receptor epsilon 1 or epsilon 2 subunit gene on NMDA receptor-mediated excitatory postsynaptic currents (NMDA EPSCs) and long-term potentiations (LTPs) at the two types of synapse in mouse hippocampal CA3 pyramidal neurons: those formed by the commissural/associational (C/A) and fimbrial (Fim) inputs. 2. Electrophysiological experiments were performed in hippocampal slices prepared from both wild-type and epsilon 1- or epsilon 2-disrupted mice using extracellular and whole-cell patch recording techniques. To assess the epsilon 1, epsilon 2 and zeta 1 subunit expression at cellular levels, we performed non-isotopic in situ hybridization with digoxigenin-labelled cRNA probes. 3. We could record EPSCs in response to the stimulations to either of the C/A and Fim afferents from a single CA3 pyramidal neuron. The epsilon 1, epsilon 2 and zeta 1 subunits were expressed together in individual CA3 neurons. 4. The epsilon 1 subunit disruption selectively reduced NMDA EPSCs and LTP in the C/A-CA3 synapse without significantly affecting those in the Fim-CA3 synapse, whereas the epsilon 2 subunit mutation diminished NMDA EPSCs and LTP in the Fim-CA3 synapse with no appreciable functional modifications in the C/A-CA3 synapse. 5. These results suggest that NMDA receptors with different subunit compositions function within a single CA3 pyramidal cell in a synapse-selective manner. |
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| Keywords |
Animals; Chimera; Electrophysiology; Hippocampus/chemistry; Hippocampus/cytology; Hippocampus/physiology; Long-Term Potentiation/physiology; Mice; Mice, Inbred C57BL; Mice, Inbred CBA; Mice, Inbred ICR; Mice, Mutant Strains/physiology; Mutagenesis/physiology; Organ Culture Techniques; Pyramidal Cells/chemistry; Pyramidal Cells/physiology; Pyramidal Cells/ultrastructure; Receptors, N-Methyl-D-Aspartate/chemistry; Receptors, N-Methyl-D-Aspartate/genetics; Receptors, N-Methyl-D-Aspartate/metabolism; Synapses/chemistry; Synapses/physiology |
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Significance
Annotations
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