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PMID:9139918
| Citation |
Boles, E, Schulte, F, Miosga, T, Freidel, K, Schlüter, E, Zimmermann, FK, Hollenberg, CP and Heinisch, JJ (1997) Characterization of a glucose-repressed pyruvate kinase (Pyk2p) in Saccharomyces cerevisiae that is catalytically insensitive to fructose-1,6-bisphosphate. J. Bacteriol. 179:2987-93 |
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| Abstract |
We have characterized the gene YOR347c of Saccharomyces cerevisiae and shown that it encodes a second functional pyruvate kinase isoenzyme, Pyk2p. Overexpression of the YOR347c/PYK2 gene on a multicopy vector restored growth on glucose of a yeast pyruvate kinase 1 (pyk1) mutant strain and could completely substitute for the PYK1-encoded enzymatic activity. PYK2 gene expression is subject to glucose repression. A pyk2 deletion mutant had no obvious growth phenotypes under various conditions, but the growth defects of a pyk1 pyk2 double-deletion strain were even more pronounced than those of a pyk1 single-mutation strain. Pyk2p is active without fructose-1,6-bisphosphate. However, overexpression of PYK2 during growth on ethanol did not cause any of the deleterious effects expected from a futile cycling between pyruvate and phosphoenolpyruvate. The results indicate that the PYK2-encoded pyruvate kinase may be used under conditions of very low glycolytic flux. |
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| Keywords |
Allosteric Regulation; Amino Acid Sequence; Animals; Base Sequence; Ethanol/metabolism; Fructosediphosphates/metabolism; Gene Deletion; Genes, Fungal; Genotype; Glucose/metabolism; Glycolysis; Isoenzymes/chemistry; Isoenzymes/genetics; Isoenzymes/metabolism; Kidney/enzymology; Kinetics; Liver/enzymology; Molecular Sequence Data; Muscle, Skeletal/enzymology; Oligodeoxyribonucleotides; Pyruvate Kinase/chemistry; Pyruvate Kinase/genetics; Pyruvate Kinase/metabolism; Rats; Recombinant Fusion Proteins/biosynthesis; Recombinant Fusion Proteins/chemistry; Recombinant Fusion Proteins/metabolism; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics; Saccharomyces cerevisiae/physiology; Sequence Homology, Amino Acid; Substrate Specificity; beta-Galactosidase/metabolism |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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