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Gross, T, Lützelberger, M, Weigmann, H, Klingenhoff, A, Shenoy, S and Käufer, NF (1997) Functional analysis of the fission yeast Prp4 protein kinase involved in pre-mRNA splicing and isolation of a putative mammalian homologue. Nucleic Acids Res. 25:1028-35
The prp4 gene of Schizosaccharomyces pombe encodes a protein kinase. A physiological substrate is not yet known. A mutational analysis of prp4 revealed that the protein consists of a short N-terminal domain, containing several essential motifs, which is followed by the kinase catalytic domain comprising the C-terminus of the protein. Overexpression of N-terminal mutations disturbs mitosis and produces elongated cells, Using a PCR approach, we isolated a putative homologue of Prp4 from human and mouse cells. The mammalian kinase domain is 53% identical to the kinase domain of Prp4. The short N-terminal domains share <20% identical amino acids, but contain conserved motifs. A fusion protein consisting of the N-terminal region from S. pombe followed by the mammalian kinase domain complements a temperature-sensitive prp4 mutation of S. pombe. Prp4 and the recombinant yeast/mouse protein kinase phosphorylate the human SR splicing factor ASF/SF2 in vitro in its RS domain.
Amino Acid Sequence; Animals; Binding Sites; DNA Primers; DNA, Complementary; Genetic Complementation Test; HeLa Cells; Humans; Mammals; Mice; Mitosis; Molecular Sequence Data; Mutagenesis, Site-Directed; Nuclear Proteins/metabolism; Protein-Serine-Threonine Kinases/genetics; Protein-Serine-Threonine Kinases/metabolism; RNA Precursors/metabolism; RNA Splicing; RNA-Binding Proteins; Rabbits; Recombinant Fusion Proteins/genetics; Recombinant Fusion Proteins/metabolism; Ribonucleoprotein, U4-U6 Small Nuclear/genetics; Ribonucleoprotein, U4-U6 Small Nuclear/metabolism; Schizosaccharomyces/enzymology; Schizosaccharomyces/genetics; Schizosaccharomyces pombe Proteins; Sequence Homology, Amino Acid
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