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PMID:9056253
| Citation |
Inoue, T, Yatsuki, H, Kusakabe, T, Joh, K, Takasaki, Y, Nikoh, N, Miyata, T and Hori, K (1997) Caenorhabditis elegans has two isozymic forms, CE-1 and CE-2, of fructose-1,6-bisphosphate aldolase which are encoded by different genes. Arch. Biochem. Biophys. 339:226-34 |
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| Abstract |
Two distinct types of cDNAs for fructose-1,6-bisphosphate (FBP) aldolase, Ce-1 and Ce-2, have been isolated from nematode Caenorhabditis elegans, and the respective recombinant aldolase isozymes, CE-1 and CE-2, have been purified and characterized. The Ce-1 and Ce-2 are 1282 and 1248 bp in total length, respectively, and both have an open reading frame of 1098 bp, which encodes 366 amino acid residues. The entire amino acid sequences deduced from Ce-1 and Ce-2 show a high degree of identity to one another and to those of vertebrate and invertebrate aldolases. The highest sequence diversity was found in the carboxyl-terminal region that corresponds to one of the isozyme group-specific sequences of vertebrate aldolase isozymes that play a role in determining isozyme-specific functions. Southern blot analysis suggests that CE-1 and CE-2 are encoded by different genes. Concerning general or kinetic properties, CE-2 is quite different from CE-1. CE-1 exhibits unique characteristics which are not identical to any aldolase isozymes previously reported, whereas CE-2 is similar to vertebrate aldolase C. These results suggest that CE-2 might preserve the properties of a progenitor aldolase with a moderate preference for FBP over fructose 1-phosphate (F1P) as a substrate, whereas CE-1 evolved to act as an intrinsic enzyme that exhibits a much broader substrate specificity than dose CE-2. |
| Links |
PubMed Online version:10.1006/abbi.1996.9813 |
| Keywords |
Amino Acid Sequence; Animals; Base Sequence; Caenorhabditis elegans/genetics; Fructose-Bisphosphate Aldolase/genetics; Gene Expression; Genes, Helminth; Isoenzymes/genetics; Kinetics; Molecular Sequence Data; Molecular Weight; Phylogeny; RNA, Messenger/genetics; Recombinant Proteins/isolation & purification; Recombinant Proteins/metabolism; Restriction Mapping; Sequence Homology, Amino Acid; Species Specificity |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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