GONUTS has been updated to MW1.31 Most things seem to be working but be sure to report problems.
PMID:9008709
| Citation |
Parton, RG, Way, M, Zorzi, N and Stang, E (1997) Caveolin-3 associates with developing T-tubules during muscle differentiation. J. Cell Biol. 136:137-54 |
|---|---|
| Abstract |
Caveolae, flask-shaped invaginations of the plasma membrane, are particularly abundant in muscle cells. We have recently cloned a muscle-specific caveolin, termed caveolin-3, which is expressed in differentiated muscle cells. Specific antibodies to caveolin-3 were generated and used to characterize the distribution of caveolin-3 in adult and differentiating muscle. In fully differentiated skeletal muscle, caveolin-3 was shown to be associated exclusively with sarcolemmal caveolae. Localization of caveolin-3 during differentiation of primary cultured muscle cells and development of mouse skeletal muscle in vivo suggested that caveolin-3 is transiently associated with an internal membrane system. These elements were identified as developing transverse-(T)-tubules by double-labeling with antibodies to the alpha 1 subunit of the dihydropyridine receptor in C2C12 cells. Ultrastructural analysis of the caveolin-3-labeled elements showed an association of caveolin-3 with elaborate networks of interconnected caveolae, which penetrated the depths of the muscle fibers. These elements, which formed regular reticular structures, were shown to be surface-connected by labeling with cholera toxin conjugates. The results suggest that caveolin-3 transiently associates with T-tubules during development and may be involved in the early development of the T-tubule system in muscle. |
| Links | |
| Keywords |
Amino Acid Sequence; Animals; Caveolin 1; Caveolin 3; Caveolins; Cell Differentiation; Cells, Cultured; Cholera Toxin; Intracellular Membranes/chemistry; Intracellular Membranes/ultrastructure; Membrane Proteins/analysis; Mice; Molecular Sequence Data; Muscle Fibers, Skeletal/chemistry; Muscle Fibers, Skeletal/ultrastructure; Muscle, Skeletal/chemistry; Muscle, Skeletal/cytology; Muscle, Skeletal/embryology; Myocardium/chemistry; Sarcolemma/chemistry |
| edit table |
Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
|---|---|---|---|---|---|---|---|---|
| edit table |
See also
References
See Help:References for how to manage references in GONUTS.
