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PMID:8979397
Citation |
Leymarie, J, Damerval, C, Marcotte, L, Combes, V and Vartanian, N (1996) Two-dimensional protein patterns of Arabidopsis wild-type and auxin insensitive mutants, axr1, axr2, reveal interactions between drought and hormonal responses. Plant Cell Physiol. 37:966-75 |
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Abstract |
In order to detect gene products involved in Arabidopsis drought adaptive strategy, 2D-PAGE protein patterns of two auxin-insensitive mutants, axr1, axr2, differentially affected in specific drought responses, were compared to the wild-type Columbia ecotype, in well-watered and drought-stressed conditions. Coupled to computer analysis of polypeptide amounts, 2D-electrophoresis revealed subtle changes in protein expression induced by progressive drought stress and/or mutations affecting the auxin response pathway. The differential protein patterns of axr1 and axr2 were consistent with their contrasting drought responses. The specific leaf and root protein patterns of axr1 showed that this mutation disrupts drought responses related to auxin regulation. In particular, the near absence of drought rhizogenesis in axr1 was associated with a root protein pattern closer to the well-watered than to the water-stressed axr2 and Columbia wild-type root protein patterns. Also, the largely different effects of axr1 and axr2 mutations suggest that they affect different pathways in auxin response. Several sets of polypeptides, whose regulation was affected by drought and/or mutation, were thus detected. These polypeptides could play a role both in the auxin and the drought response pathways. Their identification, through microsequencing, should be most informative. |
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Keywords |
Arabidopsis/genetics; Arabidopsis/metabolism; Arabidopsis Proteins; Electrophoresis, Gel, Two-Dimensional; Growth Substances; Mutation; Plant Growth Regulators/metabolism; Plant Leaves/metabolism; Plant Proteins/genetics; Plant Proteins/isolation & purification; Plant Proteins/metabolism; Plant Roots/metabolism; Water |
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Significance
Annotations
Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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References
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