PMID:8960364

Citation	Wang, L and Schultz, GA (1996) Expression of Oct-4 during differentiation of murine F9 cells. Biochem. Cell Biol. 74:579-84
Abstract	Oct-4 is a transcription factor that shares a common structural motif with members of the POU family. The mRNA for Oct-4 is found in growing oocytes and in totipotent or pluripotent cells of the early mouse embryo. Oct-4 is down-regulated in embryos during differentiation events associated with blastocyst implantation and gastrulation. Oct-4 gene expression is also down-regulated when murine embryonic stem cells or embryonal carcinoma cells are induced to differentiate in the presence of retinoic acid. A polyclonal antibody that can recognize a unique peptide sequence in the C-terminus of mouse Oct-4 has been prepared. It specifically recognizes Oct-4 protein as tested by Western blots and gel mobility shift assays. This antibody has been used to measure Oct-4 protein levels during retinoic acid induced differentiation of F9 embryonal carcinoma cells, It was observed that Oct-4 protein was abundant in undifferentiated F9 cells but decreased to levels below detection as the cells differentiated, consistent with changes in levels of expression in early embryos.
Links	PubMed
Keywords	Amino Acid Sequence; Animals; Bucladesine/pharmacology; Cell Differentiation/genetics; Cell Line; DNA-Binding Proteins/genetics; DNA-Binding Proteins/metabolism; Down-Regulation; Embryonal Carcinoma Stem Cells; Gene Expression Regulation; Mice; Molecular Sequence Data; Neoplastic Stem Cells/cytology; Octamer Transcription Factor-3; RNA, Messenger/genetics; RNA, Messenger/metabolism; Transcription Factors/genetics; Transcription Factors/metabolism; Tretinoin/pharmacology
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Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
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