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PMID:8893542

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Citation

Léon-Kloosterziel, KM, Gil, MA, Ruijs, GJ, Jacobsen, SE, Olszewski, NE, Schwartz, SH, Zeevaart, JA and Koornneef, M (1996) Isolation and characterization of abscisic acid-deficient Arabidopsis mutants at two new loci. Plant J. 10:655-61

Abstract

Novel Arabidopsis mutants with lowered levels of endogenous abscisic acid (ABA) were isolated. These were selected in a screen for germination in the presence of the gibberellin biosynthesis inhibitor paclobutrazol. Another mutant was isolated in a screen for NaCl tolerance. The ABA-deficiency was caused by two monogenic, recessive mutations, aba2 and aba3, that were both located on chromosome 1. The mutants showed a phenotype that is known to be characteristic for ABA-deficiency: a reduced seed dormancy and excessive water loss, leading to a wilty phenotype. Double mutant analysis, combining different aba mutations, indicated the leaky nature of the mutations.

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Keywords

Abscisic Acid/biosynthesis; Abscisic Acid/genetics; Arabidopsis/drug effects; Arabidopsis/genetics; Arabidopsis/radiation effects; Chromosome Mapping; Crosses, Genetic; Darkness; Dose-Response Relationship, Drug; Drug Resistance; Genes, Plant; Genes, Recessive; Germination; Light; Meiosis; Mutation; Polyethylene Glycols/pharmacology; Selection, Genetic; Sodium Chloride/pharmacology; Triazoles/pharmacology; Water/metabolism

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Annotations

Gene product Qualifier GO ID GO term name Evidence Code with/from Aspect Notes Status


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