PMID:8893027

Citation	Komatsu, H, Mori, I, Rhee, JS, Akaike, N and Ohshima, Y (1996) Mutations in a cyclic nucleotide-gated channel lead to abnormal thermosensation and chemosensation in C. elegans. Neuron 17:707-18
Abstract	The C. elegans tax-4 mutants are abnormal in multiple sensory behaviors: they fail to respond to temperature or to water-soluble or volatile chemical attractants. We show that the predicted tax-4 gene product is highly homologous to vertebrate cyclic nucleotide-gated channels. Tax-4 protein expressed in cultured cells functions as a cyclic nucleotide-gated channel. The green fluorescent protein (GFP)-tagged functional Tax-4 protein is expressed in thermosensory, gustatory, and olfactory neurons mediating all the sensory behaviors affected by the tax-4 mutations. The Tax-4::GFP fusion is partly localized at the sensory endings of these neurons. The results suggest that a cyclic nucleotide-gated channel is required for thermosensation and chemosensation and that cGMP is an important intracellular messenger in C. elegans sensory transduction.
Links	PubMed
Keywords	Amino Acid Sequence; Animals; Caenorhabditis elegans/genetics; Caenorhabditis elegans/physiology; Caenorhabditis elegans Proteins; Cattle; Chemotaxis; Cyclic GMP/metabolism; Drosophila; Drosophila Proteins; Genes, Helminth; Green Fluorescent Proteins; Humans; Ion Channels/biosynthesis; Ion Channels/chemistry; Ion Channels/genetics; Luminescent Proteins/biosynthesis; Macromolecular Substances; Membrane Potentials; Molecular Sequence Data; Mutagenesis; Neurons, Afferent/physiology; Potassium Channels/chemistry; Protein Structure, Secondary; Rats; Recombinant Fusion Proteins/biosynthesis; Second Messenger Systems; Sequence Homology, Amino Acid; Shaker Superfamily of Potassium Channels; Temperature
edit table

Annotations

Gene product	Qualifier	GO ID	GO term name	Evidence Code	with/from	Aspect	Notes	Status
edit table

References

See Help:References for how to manage references in GONUTS.