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PMID:8843944
| Citation |
Biermann, B, Randall, SK and Crowell, DN (1996) Identification and isoprenylation of plant GTP-binding proteins. Plant Mol. Biol. 31:1021-8 |
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| Abstract |
To identify isoprenylated plant GTP-binding proteins, Arabidopsis thaliana and Nicotiana tabacum cDNA expression libraries were screened for cDNA-encoded proteins capable of binding [32P]GTP in vitro. ATGB2, an Arabidopsis homologue of the GTP-binding protein Rab2, was found to bind GTP in vitro and to be a substrate for a geranylgeranyl:protein transferase (GGTase) present in plant extracts. The carboxyl terminus of this protein contains a -GCCG sequence, which has not previously been shown to be recognized by any prenyl:protein transferase (PTase), but which most closely resembles that isoprenylated by the type II GGTase (-XXCC, -XCXC, or -CCXX). In vitro geranylgeranylation of an Arabidopsis Rab1 protein containing a carboxyl-terminal-CCGQ sequence confirmed the presence of a type II GGTase-like activity in plant extracts. Several other proteins were also identified by in vitro GTP binding, including Arabidopsis and tobacco homologues of Rab11, ARF (ADP-ribosylation factor) and Sar proteins, as well as a novel 22 kDa Arabidopsis protein (ATG81). This 22 kDa protein had consensus GTP-binding motifs and bound GTP with high specificity, but its structure was not closely related to that of any known GTP-binding protein (it most resembled proteins within the ARF/Sar and G protein alpha-subunit superfamilies). |
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| Keywords |
Amino Acid Sequence; Arabidopsis/genetics; DNA, Complementary/genetics; Diterpenes/metabolism; GTP-Binding Proteins/classification; GTP-Binding Proteins/genetics; GTP-Binding Proteins/metabolism; Gene Library; Guanosine Triphosphate/metabolism; Molecular Sequence Data; Plant Proteins/genetics; Plant Proteins/metabolism; Plants, Toxic; Protein Prenylation; RNA, Plant/genetics; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Tobacco/genetics; Transferases/metabolism; rab2 GTP-Binding Protein |
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Significance
Annotations
| Gene product | Qualifier | GO ID | GO term name | Evidence Code | with/from | Aspect | Notes | Status |
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